ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -
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kwic search for under out of >500 occurrences
321496 occurrences (No.66 in the rank) during 5 years in the PubMed. [cache]
286) To sustain the β-cell-like phenotype and investigate the effect of three-dimensional (3D) conformation on the differentiation of hAFSCs, Pdx1-transduced cells were encapsulated in alginate and cultured long-term under serum-free conditions.
2015 Journal of tissue engineering and regenerative medicine
* Pdx1 and controlled culture conditions induced differentiation of human amniotic fluid-derived stem cells to insulin-producing clusters.
- This study investigated the differentiation of human amniotic fluid-derived stem cells (hAFSCs) into insulin-producing clusters in vitro. Adenovirally-delivered mouse Pdx1 (Ad-Pdx1) induced human Pdx1 expression in hAFSCs and enhanced the coordinated expression of downstream β-cell markers. When Ad-Pdx1-transduced hAFSCs were sequentially treated with activin A, bFGF and nicotinamide and the culture plate surface coated with poly-l-ornithine, the expression of islet-associated human mRNAs for Pdx1, Pax6, Ngn3 and insulin was increased. C-peptide ELISA confirmed that Ad-Pdx1-transduced hAFSCs processed and secreted insulin in a manner consistent with that pathway in pancreatic β-cells. To sustain the β-cell-like phenotype and investigate the effect of three-dimensional (3D) conformation on the differentiation of hAFSCs, Pdx1-transduced cells were encapsulated in alginate and cultured long-term under serum-free conditions. Over 2 weeks, partially differentiated hAFSC clusters increased in size and increased insulin secretion. Taken together, these data demonstrate that ectopic Pdx1 expression initiates pancreatic differentiation in hAFSCs and that a β-cell-like phenotype can be augmented by culture conditions that mimic the stromal components and 3D geometry associated with pancreatic islets.
=>以下の, (治療, 試練, 刑罰)を受けて, 下部の, の下に, ・・の下に
Overview of adj under
The adj under has 2 senses (first 2 from tagged texts)
1. (2) nether, under -- (located below or beneath something else; "nether garments"; "the under
parts of a machine")
2. (1) under -- (lower in rank, power, or authority; "an under secretary")
Overview of adv under
The adv under has 8 senses (first 1 from tagged texts)
1. (2) under -- (down to defeat, death, or ruin; "their competitors went under")
2. under -- (through a range downward; "children six and under will be admitted free")
3. under -- (into unconsciousness; "this will put the patient under")
4. under -- (in or into a state of subordination or subjugation; "we must keep our disappointment
under")
5. under -- (below some quantity or limit; "fifty dollars or under")
6. under -- (below the horizon; "the sun went under")
7. under -- (down below; "get under quickly!")
8. under, below -- (further down; "see under for further discussion")
--- WordNet end ---