kwic
total
total
right
off
ON
320
1
100
500
strict
ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -
return
kwic search for total out of >500 occurrences
296134 occurrences (No.80 in the rank) during 5 years in the PubMed. [no cache] 500 found
--- ABSTRACT ---
--- ABSTRACT END ---
[frequency of next (right) word to total]
296134 occurrences (No.80 in the rank) during 5 years in the PubMed. [no cache] 500 found
| 320) In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. |
| PMID:23349081 DOI:10.1002/term.1682 |
| 2015 Journal of tissue engineering and regenerative medicine |
| * An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering. |
| - Regenerative medicine is targeted to improve, restore or replace damaged tissues or organs using a combination of cells, materials and growth factors. Both tissue engineering and developmental biology currently deal with the process of tissue self-assembly and extracellular matrix (ECM) deposition. In this investigation, additive manufacturing (AM) with a multihead deposition system (MHDS) was used to fabricate three-dimensional (3D) cell-printed scaffolds using layer-by-layer (LBL) deposition of polycaprolactone (PCL) and chondrocyte cell-encapsulated alginate hydrogel. Appropriate cell dispensing conditions and optimum alginate concentrations for maintaining cell viability were determined. In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. PCL-alginate gels containing transforming growth factor-β (TGFβ) showed higher ECM formation. The 3D cell-printed scaffolds of PCL-alginate gel were implanted in the dorsal subcutaneous spaces of female nude mice. Histochemical [Alcian blue and haematoxylin and eosin (H&E) staining] and immunohistochemical (type II collagen) analyses of the retrieved implants after 4 weeks revealed enhanced cartilage tissue and type II collagen fibril formation in the PCL-alginate gel (+TGFβ) hybrid scaffold. In conclusion, we present an innovative cell-printed scaffold for cartilage regeneration fabricated by an advanced bioprinting technology. |
[
right
kwic]
(1)187  of |
(14)3 adhesive |
(27)2 BGP |
(40)2 organic |
| (2)21 length |
(15)3 annual |
(28)2 DNA, |
(41)2 oxidant |
| (3)13 number |
(16)3 delivered |
(29)2 activity |
(42)2 pleural |
| (4)7 antioxidant |
(17)3 knee |
(30)2 arch |
(43)2 protein, |
| (5)7 protein |
(18)3 length, |
(31)2 biomass |
(44)2 repetitions |
| (6)5 body |
(19)3 optical |
(32)2 collagen |
(45)2 scores |
| (7)5 cholesterol |
(20)3 or |
(33)2 daily |
(46)2 snack |
| (8)5 dose |
(21)3 parenteral |
(34)2 dietary |
(47)2 soluble |
| (9)5 score |
(22)3 polyphenols |
(35)2 exercise |
(48)2 stress |
| (10)4 HG |
(23)3 population |
(36)2 genome |
(49)2 tumor |
| (11)4 amount |
(24)3 sample |
(37)2 health-care |
(50)2 volume |
| (12)4 and |
(25)3 suspended |
(38)2 islet |
|
| (13)4 cell |
(26)2 248 |
(39)2 mitochondrial |
add keyword
--- WordNet output for total ---