ELIZA

return kwic search for time out of >500 occurrences
357929 occurrences (No.51 in the rank) during 5 years in the PubMed. [cache]

228) ITGAV mRNA expression levels were measured in BEAS-2B cells using real-time polymerase chain reaction.

--- ABSTRACT ---

PMID:24023007 DOI:10.1002/tox.21906

2015 Environmental toxicology

* Genome wide identification and expression profile in epithelial cells exposed to TiO₂ particles.

- Environmental particles are believed to provoke airway inflammation in susceptible individuals by stimulating epithelial cells to release mediators that exacerbate lung diseases. Here, we sought to identify genes expressed throughout the genome by epithelial cells stimulated with TiO2 particles. A human bronchial epithelial cell line, BEAS-2B, was stimulated with or without 40 µg TiO2 for 2 h. RNA was purified from cells and subjected to microarray analysis. Genes exhibiting more than a twofold change in RNA expression were selected. Candidate genes were then analyzed using bioinformatics tools, including pathway, ontology, and network analyses. ITGAV mRNA expression levels were measured in BEAS-2B cells using real-time polymerase chain reaction. Among 37,803 genes, 92 genes displayed more than a twofold change in mRNA levels according to the microarray analysis; 87 genes were upregulated while five genes were downregulated. The 92 genes were classified based on functional annotation using a protein information resource database search for biological processes and a pathway search using the KEGG pathway database. These genes are related to macromolecule biosynthesis, metabolic processes and, in particular, RNA metabolism. When genes with more than a threefold change were analyzed, KIF11, ITGAV, SEMA3C, IBTK, and DEK were selected as candidate genes induced by TiO2 -stimulated BEAS-2B cells. To validate these results, BEAS-2B cells stimulated with 40 µg TiO2 expressed threefold higher ITGAV mRNA levels compared to those without TiO2 particle stimulation. We conclude that KIF11, ITGAV, SEMA3C, IBTK, and DEK are candidate genes expressed by epithelial cells when stimulated with TiO2 particles.

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(1)48 null	(14)6 periods	(27)3 frames	(40)2 needed
(2)40 of	(15)5 frame	(28)3 on	(41)2 parameters
(3)34 and	(16)5 from	(29)3 quantitative	(42)2 pressure
(4)34 to	(17)5 period	(30)3 since	(43)2 required
(5)14 in	(18)5 physical	(31)3 taken	(44)2 spacing
(6)12 PCR	(19)5 the	(32)2 1	(45)2 spent
(7)11 was	(20)4 a	(33)2 1)	(46)2 targets
(8)10 points	(21)4 by	(34)2 PCR,	(47)2 test
(9)9 for	(22)4 or	(35)2 data	(48)2 that
(10)8 course	(23)4 reverse	(36)2 even	(49)2 use
(11)8 polymerase	(24)4 series	(37)2 interval	(50)2 used
(12)6 RT-PCR	(25)3 aerobic	(38)2 it	(51)2 will
(13)6 manner	(26)3 consuming	(39)2 maintain

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--- WordNet output for time --- =>1.時代, 季節, 歳月, 当時, 現代, 一生, 年季, 就業時間, 期間, 時刻, 時点, 機会, 拍子, 速度, 何度, 何回, 時, 時間, 回, 2.時間[速度]を測定する, 時間を決める, (装置などの)タイミングを調節する Overview of noun time The noun time has 10 senses (first 9 from tagged texts) 1. (219) time, clip -- (an instance or single occasion for some event; "this time he succeeded"; "he called four times"; "he could do ten at a clip") 2. (160) time -- (a period of time considered as a resource under your control and sufficient to accomplish something; "take time to smell the roses"; "I didn't have time to finish"; "it took more than half my time") 3. (114) time -- (an indefinite period (usually marked by specific attributes or activities); "he waited a long time"; "the time of year for planting"; "he was a great actor in his time") 4. (43) time -- (a suitable moment; "it is time to go") 5. (36) time -- (the continuum of experience in which events pass from the future through the present to the past) 6. (10) time -- (a person's experience on a particular occasion; "he had a time holding back the tears"; "they had a good time together") 7. (7) clock time, time -- (a reading of a point in time as given by a clock; "do you know what time it is?"; "the time is 10 o'clock") 8. (5) fourth dimension, time -- (the fourth coordinate that is required (along with three spatial dimensions) to specify a physical event) 9. (1) meter, metre, time -- (rhythm as given by division into parts of equal duration) 10. prison term, sentence, time -- (the period of time a prisoner is imprisoned; "he served a prison term of 15 months"; "his sentence was 5 to 10 years"; "he is doing time in the county jail") Overview of verb time The verb time has 5 senses (first 3 from tagged texts) 1. (4) clock, time -- (measure the time or duration of an event or action or the person who performs an action in a certain period of time; "he clocked the runners") 2. (3) time -- (assign a time for an activity or event; "The candidate carefully timed his appearance at the disaster scene") 3. (1) time -- (set the speed, duration, or execution of; "we time the process to manufacture our cars very precisely") 4. time -- (regulate or set the time of; "time the clock") 5. time -- (adjust so that a force is applied and an action occurs at the desired time; "The good player times his swing so as to hit the ball squarely") --- WordNet end ---