ELIZA

return kwic search for time out of >500 occurrences
357929 occurrences (No.51 in the rank) during 5 years in the PubMed. [no cache] 500 found

180) The RNA detected positive (n = 28, 40%) using real-time PCR was subjected to conventional PCR for the amplification of the fusion and nucleoprotein genes.

--- ABSTRACT ---

PMID:23590481 DOI:10.1111/tbed.12089

2015 Transboundary and emerging diseases

* Molecular characterization of peste des petits ruminants viruses from outbreaks caused by unrestricted movements of small ruminants in pakistan.

- Peste des petits ruminants (PPR) is an endemic disease of small ruminants, and vaccination has been the method of control but outbreaks are continuously occurring in Pakistan. The following study presents a detailed investigation of an outbreak, suspected to be PPR, probably introduced by PPRV-infected sheep and goats from Sindh Province (north-west) to Punjab Province (central) of Pakistan during the flood relief campaign in 2011. A total of 70 serum samples from 28 different flocks were tested with competitive ELISA (H antibodies), which detected 24 (34.2%) samples positive for PPRV antibodies. Nasal swabs and faeces were tested with immunocapture ELISA (N antigen), which detected 18 (25.7%) samples positive for PPRV antigen. The RNA detected positive (n = 28, 40%) using real-time PCR was subjected to conventional PCR for the amplification of the fusion and nucleoprotein genes. Sequencing of both genes and subsequent phylogenetic analysis indicated the grouping of all the sequences to be in lineage IV along with other Asian isolates of PPRV. However, sequences of both genes were divided into two groups within lineage IV. One group of viruses clustered with previously characterized Pakistani isolates, whereas the other group was distinctly clustered with isolates from the Middle East or India. The sequence identity indicated the introduction of at least one population of PPRV from a different source and circulation in the local flocks of small ruminants, which emphasized the need to obtain health clearance certificate before movement of animals. The results of this study provide baseline data for the genetic characterization of different PPRV populations in Pakistan.

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(1)48 null	(14)6 periods	(27)3 frames	(40)2 needed
(2)40 of	(15)5 frame	(28)3 on	(41)2 parameters
(3)34 and	(16)5 from	(29)3 quantitative	(42)2 pressure
(4)34 to	(17)5 period	(30)3 since	(43)2 required
(5)14 in	(18)5 physical	(31)3 taken	(44)2 spacing
(6)12 PCR	(19)5 the	(32)2 1	(45)2 spent
(7)11 was	(20)4 a	(33)2 1)	(46)2 targets
(8)10 points	(21)4 by	(34)2 PCR,	(47)2 test
(9)9 for	(22)4 or	(35)2 data	(48)2 that
(10)8 course	(23)4 reverse	(36)2 even	(49)2 use
(11)8 polymerase	(24)4 series	(37)2 interval	(50)2 used
(12)6 RT-PCR	(25)3 aerobic	(38)2 it	(51)2 will
(13)6 manner	(26)3 consuming	(39)2 maintain

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--- WordNet output for time --- =>1.時代, 季節, 歳月, 当時, 現代, 一生, 年季, 就業時間, 期間, 時刻, 時点, 機会, 拍子, 速度, 何度, 何回, 時, 時間, 回, 2.時間[速度]を測定する, 時間を決める, (装置などの)タイミングを調節する Overview of noun time The noun time has 10 senses (first 9 from tagged texts) 1. (219) time, clip -- (an instance or single occasion for some event; "this time he succeeded"; "he called four times"; "he could do ten at a clip") 2. (160) time -- (a period of time considered as a resource under your control and sufficient to accomplish something; "take time to smell the roses"; "I didn't have time to finish"; "it took more than half my time") 3. (114) time -- (an indefinite period (usually marked by specific attributes or activities); "he waited a long time"; "the time of year for planting"; "he was a great actor in his time") 4. (43) time -- (a suitable moment; "it is time to go") 5. (36) time -- (the continuum of experience in which events pass from the future through the present to the past) 6. (10) time -- (a person's experience on a particular occasion; "he had a time holding back the tears"; "they had a good time together") 7. (7) clock time, time -- (a reading of a point in time as given by a clock; "do you know what time it is?"; "the time is 10 o'clock") 8. (5) fourth dimension, time -- (the fourth coordinate that is required (along with three spatial dimensions) to specify a physical event) 9. (1) meter, metre, time -- (rhythm as given by division into parts of equal duration) 10. prison term, sentence, time -- (the period of time a prisoner is imprisoned; "he served a prison term of 15 months"; "his sentence was 5 to 10 years"; "he is doing time in the county jail") Overview of verb time The verb time has 5 senses (first 3 from tagged texts) 1. (4) clock, time -- (measure the time or duration of an event or action or the person who performs an action in a certain period of time; "he clocked the runners") 2. (3) time -- (assign a time for an activity or event; "The candidate carefully timed his appearance at the disaster scene") 3. (1) time -- (set the speed, duration, or execution of; "we time the process to manufacture our cars very precisely") 4. time -- (regulate or set the time of; "time the clock") 5. time -- (adjust so that a force is applied and an action occurs at the desired time; "The good player times his swing so as to hit the ball squarely") --- WordNet end ---