ELIZA

return kwic search for through out of >500 occurrences
437431 occurrences (No.38 in the rank) during 5 years in the PubMed. [no cache] 500 found

9) Taken together, our findings indicate that AJUBA negatively regulates YAP activity through the LATS family, and inactivation of AJUBA is a novel key mechanism in MM cell proliferation.

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PMID:24336325 DOI:10.1038/onc.2013.528

2015 Oncogene

* LIM-domain protein AJUBA suppresses malignant mesothelioma cell proliferation via Hippo signaling cascade.

- Malignant mesothelioma (MM) is one of the most aggressive neoplasms usually associated with asbestos exposure and is highly refractory to current therapeutic modalities. MMs show frequent activation of a transcriptional coactivator Yes-associated protein (YAP), which is attributed to the neurofibromatosis type 2 (NF2)-Hippo pathway dysfunction, leading to deregulated cell proliferation and acquisition of a malignant phenotype. However, the whole mechanism of disordered YAP activation in MMs has not yet been well clarified. In the present study, we investigated various components of the NF2-Hippo pathway, and eventually found that MM cells frequently showed downregulation of LIM-domain protein AJUBA, a binding partner of large tumor suppressor type 2 (LATS2), which is one of the last-step kinases of the NF2-Hippo pathway. Although loss of AJUBA expression was independent of the alteration status of other Hippo pathway components, MM cell lines with AJUBA inactivation showed a more dephosphorylated (activated) level of YAP. Immunohistochemical analysis showed frequent downregulation of AJUBA in primary MMs, which was associated with YAP constitutive activation. We found that AJUBA transduction into MM cells significantly suppressed promoter activities of YAP-target genes, and the suppression of YAP activity by AJUBA was remarkably canceled by knockdown of LATS2. In connection with these results, transduction of AJUBA-expressing lentivirus significantly inhibited the proliferation and anchorage-independent growth of the MM cells that harbored ordinary LATS family expression. Taken together, our findings indicate that AJUBA negatively regulates YAP activity through the LATS family, and inactivation of AJUBA is a novel key mechanism in MM cell proliferation.

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[frequency of next (right) word to through]

(1)113 the	(12)3 biological	(23)2 direct	(34)2 low
(2)51 a	(13)3 both	(24)2 evidence	(35)2 maternal
(3)11 an	(14)3 cortical	(25)2 focus	(36)2 paracrine
(4)10 which	(15)3 histological	(26)2 four	(37)2 promoting
(5)5 their	(16)3 infancy	(27)2 human	(38)2 rat
(6)4 activation	(17)3 interaction	(28)2 information,	(39)2 reward
(7)4 its	(18)3 simulation	(29)2 inhibition	(40)2 skin
(8)4 median	(19)2 2012	(30)2 interactions	(41)2 such
(9)4 three	(20)2 August	(31)2 international	(42)2 these
(10)4 use	(21)2 PCR-based	(32)2 intersection
(11)3 analysis	(22)2 different	(33)2 interviews

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--- WordNet output for through --- =>直通の, のために, の間じゅう, 終わって, を貫いて, の中を通って, のいたるところに, を通じて Overview of adj through The adj through has 2 senses (first 1 from tagged texts) 1. (4) done, through, through with -- (having finished or arrived at completion; "certain to make history before he's done"; "it's a done deed"; "after the treatment, the patient is through except for follow-up"; "almost through with his studies") 2. through -- ((of a route or journey etc.) continuing without requiring stops or changes; "a through street"; "a through bus"; "through traffic") Overview of adv through The adv through has 5 senses (first 3 from tagged texts) 1. (5) through -- (from beginning to end; "read this book through") 2. (2) through -- (over the whole distance; "this bus goes through to New York") 3. (1) through -- (to completion; "think this through very carefully!") 4. through -- (in diameter; "this cylinder measures 15 inches through") 5. through, through and through -- (throughout the entire extent; "got soaked through in the rain"; "I'm frozen through"; "a letter shot through with the writer's personality"; "knew him through and through"; "boards rotten through and through") --- WordNet end ---