ELIZA

return kwic search for through out of >500 occurrences
437431 occurrences (No.38 in the rank) during 5 years in the PubMed. [no cache] 500 found

242) These data show that ESC-derived intestinal organoids can be derived in large numbers, contain distinct ISC types and can be used to screen for agents that promote cell differentiation through different lineage pathways.

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PMID:24115167 DOI:10.1002/mc.22089

2015 Molecular carcinogenesis

* Development of intestinal organoids as tissue surrogates: cell composition and the epigenetic control of differentiation.

- Intestinal organoids are multicellular crypt-like structures that can be derived from adult intestinal stem cells (ISCs), embryonic stem cells (ESCs) or induced pluripotent stem cells (IPSCs). Here we show that intestinal organoids generated from mouse ESCs were enriched in ISCs and early progenitors. Treatment of these organoids with a γ-secretase inhibitor increased Math1 and decreased Hes1 expression, indicating Notch signaling regulates ISC differentiation in these organoids. Lgr5 and Tert positive ISCs constituted approximately 10% and 20% of the organoids. As found in native tissue, Lgr5 and Tert expressing cells resolved into two discreet populations, which were stable over time. Intestinal organoids derived from cancer-prone Apc(Min/+) mice showed similar numbers of ISCs, but had reduced Math1 expression, indicating a suppressed secretory cell differentiation potential (as found in intestinal tissue). Apc(Min/+) organoids were used to screen epigenetically active compounds for those that increased Math1 expression and organoid differentiation (including HDAC inhibitors, Sirtuin (SIRT) modulators and methyltransferase inhibitors). Broad-spectrum HDAC inhibitors increased both Math1 and Muc2 expression, indicating an ability to promote the suppressed secretory cell differentiation pathway. Other epigenetic compounds had a diverse impact on cell differentiation, with a strong negative correlation between those that activated the secretory marker Muc2 and those that activated the absorptive cell marker Fabp2. These data show that ESC-derived intestinal organoids can be derived in large numbers, contain distinct ISC types and can be used to screen for agents that promote cell differentiation through different lineage pathways.

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(1)113 the	(12)3 biological	(23)2 direct	(34)2 low
(2)51 a	(13)3 both	(24)2 evidence	(35)2 maternal
(3)11 an	(14)3 cortical	(25)2 focus	(36)2 paracrine
(4)10 which	(15)3 histological	(26)2 four	(37)2 promoting
(5)5 their	(16)3 infancy	(27)2 human	(38)2 rat
(6)4 activation	(17)3 interaction	(28)2 information,	(39)2 reward
(7)4 its	(18)3 simulation	(29)2 inhibition	(40)2 skin
(8)4 median	(19)2 2012	(30)2 interactions	(41)2 such
(9)4 three	(20)2 August	(31)2 international	(42)2 these
(10)4 use	(21)2 PCR-based	(32)2 intersection
(11)3 analysis	(22)2 different	(33)2 interviews

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--- WordNet output for through --- =>直通の, のために, の間じゅう, 終わって, を貫いて, の中を通って, のいたるところに, を通じて Overview of adj through The adj through has 2 senses (first 1 from tagged texts) 1. (4) done, through, through with -- (having finished or arrived at completion; "certain to make history before he's done"; "it's a done deed"; "after the treatment, the patient is through except for follow-up"; "almost through with his studies") 2. through -- ((of a route or journey etc.) continuing without requiring stops or changes; "a through street"; "a through bus"; "through traffic") Overview of adv through The adv through has 5 senses (first 3 from tagged texts) 1. (5) through -- (from beginning to end; "read this book through") 2. (2) through -- (over the whole distance; "this bus goes through to New York") 3. (1) through -- (to completion; "think this through very carefully!") 4. through -- (in diameter; "this cylinder measures 15 inches through") 5. through, through and through -- (throughout the entire extent; "got soaked through in the rain"; "I'm frozen through"; "a letter shot through with the writer's personality"; "knew him through and through"; "boards rotten through and through") --- WordNet end ---