ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for study, out of >500 occurrences
300943 occurrences (No.75 in the rank) during 5 years in the PubMed. [cache]
11) In the present study, we aimed to investigate the mechanisms of dentin matrix extract-induced osteoclastogenesis in mouse bone marrow macrophages (BMMs).
--- ABSTRACT ---
PMID:24366403 DOI:10.1007/s10266-013-0140-3
2015 Odontology
* TGF-β in dentin matrix extract induces osteoclastogenesis in vitro.
- Previously, we have demonstrated that the extracellular matrix from dentin affects osteoclastic activity in co-culture between osteoclast and osteoblast-rich fraction from mouse marrow cells. In the present study, we aimed to investigate the mechanisms of dentin matrix extract-induced osteoclastogenesis in mouse bone marrow macrophages (BMMs). Dentin proteins were extracted from bovine incisor root dentin using 0.6 M HCl. BMMs were cultured in α-MEM containing macrophage colony-stimulating factor/receptor activator of nuclear factor kappa-B ligand in the presence or absence of dentin matrix extract. Tartrate-resistant acid phosphatase (TRAP)-positive cell number, total TRAP activity, and the mRNA levels of osteoclast-related genes, assayed by real-time RT-PCR, were determined as markers of osteoclastogenesis. A neutralizing antibody against transforming growth factor-β1 (TGF-β1), SB431542, a TGF-β receptor inhibitor, and ELISA were used to determine the role of TGF-β1. We observed increases in TRAP-positive cell number, TRAP activity, and the mRNA levels of osteoclast-related genes of BMMs cultured with dentin extract. The use of a neutralizing antibody against TGF-β1 or SB431542 inhibited the inductive effect of dentin extract, suggesting TGF-β1 involvement. The addition of exogenous TGF-β1, but not bone morphogenic protein-2, also increased osteoclastogenesis, corresponding to the ELISA determination of TGF-β1 in the dentin extract. In conclusion, our results indicate that proteins from dentin matrix have an inductive effect in osteoclastogenesis, which is mediated, in part, by TGF-β1.
--- ABSTRACT END ---
[
right
kwic]
[frequency of next (right) word to study,]
(1)219 we (6)6 two (11)3 to (16)2 human
(2)85 the (7)4 which (12)2 24 (17)2 in
(3)22 a (8)3 DNA (13)2 an (18)2 serum
(4)11 and (9)3 therefore, (14)2 effects (19)2 six
(5)8 it (10)3 three (15)2 further

add keyword

--- WordNet output for study, --- --- WordNet end ---