* Field application of MALDI-TOF MS on mosquito larvae identification.
- In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an efficient tool for arthropod identification. Its application for field monitoring of adult mosquitoes was demonstrated, but identification of larvae has been limited to laboratory-reared specimens. Study aim was to test the success of MALDI-TOF MS in correctly identifying mosquito larvae collected in the field. Collections were performed at 13 breeding sites in urban areas of Marseille, a city in the South of France. A total of 559 larvae were collected. Of these, 73 were accurately morphologically identified, with confirmation either by molecular identification (n = 31) or analysis with MALDI-TOF MS (n = 31) and 11 were tested using both methods. The larvae identified belonged to six species including Culiseta longiareolata, Culex pipiens pipiens, Culex hortensis, Aedes albopictus, Ochlerotatus caspius and Anopheles maculipennis. A high intra-species reproducibility and inter-species specificity of whole larva MS spectra was obtained and was independent of breeding site. More than 92% of the remaining 486 larvae were identified in blind tests against the MS spectra database. Identification rates were lower for early and pupal stages, which is attributed to lower protein abundance and metamorphosis, respectively. The suitability of MALDI-TOF MS for mosquito larvae identification from the field has been confirmed.
=>1.〜を勉強[研究]する, 学ぶ, 調べる, 2.調査, 勉強, 3.書斎, 勉強する
Overview of noun study
The noun study has 10 senses (first 8 from tagged texts)
1. (90) survey, study -- (a detailed critical inspection)
2. (17) study, work -- (applying the mind to learning and understanding a subject (especially by
reading); "mastering a second language requires a lot of work"; "no schools offer graduate study in
interior design")
3. (6) report, study, written report -- (a written document describing the findings of some
individual or group; "this accords with the recent study by Hill and Dale")
4. (6) study -- (a state of deep mental absorption; "she is in a deep study")
5. (6) study -- (a room used for reading and writing and studying; "he knocked lightly on the closed
door of the study")
6. (3) discipline, subject, subject area, subject field, field, field of study, study, bailiwick --
(a branch of knowledge; "in what discipline is his doctorate?"; "teachers should be well trained in
their subject"; "anthropology is the study of human beings")
7. (2) sketch, study -- (preliminary drawing for later elaboration; "he made several studies before
starting to paint")
8. (1) cogitation, study -- (attentive consideration and meditation; "after much cogitation he
rejected the offer")
9. study -- (someone who memorizes quickly and easily (as the lines for a part in a play); "he is a
quick study")
10. study -- (a composition intended to develop one aspect of the performer's technique; "a study in
spiccato bowing")
Overview of verb study
The verb study has 6 senses (first 6 from tagged texts)
1. (73) analyze, analyse, study, examine, canvass, canvas -- (consider in detail and subject to an
analysis in order to discover essential features or meaning; "analyze a sonnet by Shakespeare";
"analyze the evidence in a criminal trial"; "analyze your real motives")
2. (17) study -- (be a student; follow a course of study; be enrolled at an institute of learning)
3. (15) study, consider -- (give careful consideration to; "consider the possibility of moving")
4. (13) learn, study, read, take -- (be a student of a certain subject; "She is reading for the bar
exam")
5. (5) study, hit the books -- (learn by reading books; "He is studying geology in his room"; "I
have an exam next week; I must hit the books now")
6. (2) study, meditate, contemplate -- (think intently and at length, as for spiritual purposes; "He
is meditating in his study")
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