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38) This method can be used to identify optimal decellularization protocols for recellularization of three-dimensional tissue-scaffolds with embryonic stem cells and other tissue-specific cell types.
2015 Journal of tissue engineering and regenerative medicine
* Dewaxed ECM: A simple method for analyzing cell behaviour on decellularized extracellular matrices.
- Decellularization techniques have been used on a wide variety of tissues to create cell-seedable scaffolds for tissue engineering. Finding a suitable decellularization protocol for a certain type of tissue can be laborious, especially when organ perfusion devices are needed. In this study, we report a quick and simple method for comparing decellularization protocols combining the use of paraffin slices and two-dimensional cell cultures. We developed three decellularization protocols for adult murine kidney that yielded decellularized extracellular matrices (ECMs) with varying histological properties. The resulting paraffin-embedded ECM slices were deparaffinized and reseeded with murine embryonic stem cells (mESCs). We analyzed cell attachment four days post seeding via determination of cell numbers, and used quantitative Real-Time PCR 13 days post seeding to measure gene expression levels of two genes associated with renal development, Pax2 and Pou3f3. The three decellularization protocols produced kidney-matrices that showed clearly distinguishable results. We demonstrated that formerly paraffin-embedded decellularized ECMs can effectively influence differentiation of stem cells. This method can be used to identify optimal decellularization protocols for recellularization of three-dimensional tissue-scaffolds with embryonic stem cells and other tissue-specific cell types.
=>特有の, 特効薬, 明確な, 特定の, 種の, はっきりした
Overview of noun specific
The noun specific has 2 senses (no senses from tagged texts)
1. particular, specific -- (a fact about some part (as opposed to general); "he always reasons from
the particular to the general")
2. specific -- (a medicine that has a mitigating effect on a specific disease; "quinine is a
specific for malaria")
Overview of adj specific
The adj specific has 4 senses (first 2 from tagged texts)
1. (39) specific -- ((sometimes followed by `to') applying to or characterized by or distinguishing
something particular or special or unique; "rules with specific application"; "demands specific to
the job"; "a specific and detailed account of the accident")
2. (5) specific -- (stated explicitly or in detail; "needed a specific amount")
3. specific -- (relating to or distinguishing or constituting a taxonomic species; "specific
characters")
4. specific -- (being or affecting a disease produced by a particular microorganism or condition;
used also of stains or dyes used in making microscope slides; "quinine is highly specific for
malaria"; "a specific remedy"; "a specific stain is one having a specific affinity for particular
structural elements")
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