367) Although wogonin expressed only weak inhibitory effect on LPS-/INF-γ-induced phosphorylation of Janus kinase-2 (Jak-2) and tyrosine kinase (Tyk)-2, it significantly attenuated the phosphorylation of Jak-1 and Jak-3. |
PMID:23592745 DOI:10.1177/0748233713485886 |
2015 Toxicology and industrial health |
* Protective effect of wogonin on proinflammatory cytokine generation via Jak1/3-STAT1/3 pathway in lipopolysaccharide stimulated BV2 microglial cells. |
- Wogonin is a flavonoid compound which exhibits antioxidation, anti-inflammation, neuroprotection, and antitumorgenesis functions. However, the mechanism of how wogonin reduces proinflammatory cytokine generation in activated microglia is unclear. At present, we found wogonin inhibited lipopolysaccharide (LPS)-/interferon-γ (INF-γ)-induced generation of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Wogonin exhibited parallel inhibition on LPS-/INF-γ-induced expression of IL-6 and TNF-α messenger RNA at the same concentration range. LPS-/INF-γ-induced phosphorylation of signal transduction and transcription 1 and 3 (STAT1/3) were also inhibited by wogonin. Although wogonin expressed only weak inhibitory effect on LPS-/INF-γ-induced phosphorylation of Janus kinase-2 (Jak-2) and tyrosine kinase (Tyk)-2, it significantly attenuated the phosphorylation of Jak-1 and Jak-3. These results indicated that the blockade of IL-6 and TNF-α production by wogonin in LPS-/INF-γ-stimulated BV2 microglial cells was attributed mainly to the interference in Jak-1/-3-STAT1/3 signaling pathway. |
(1)88 higher | (13)8 improved | (25)3 (P<0.05) | (39)2 impaired |
(2)43 lower | (14)6 correlated | (27)3 affect | (40)2 increases |
(3)39 increased | (15)6 elevated | (28)3 better | (41)2 induced |
(4)35 reduced | (16)6 fewer | (29)3 increase | (42)2 larger |
(5)32 associated | (17)6 less | (30)3 worse | (43)2 predicted |
(6)17 decreased | (18)5 enhanced | (31)2 (P | (44)2 reduce |
(7)13 in | (19)5 inhibited | (32)2 (p | (45)2 shorter |
(8)13 more | (20)4 affected | (34)2 altered | (46)2 smaller |
(9)10 greater | (21)4 attenuated | (35)2 by | (47)2 suppressed |
(10)10 related | (22)4 between | (36)2 compared | (48)2 to |
(11)9 *null* | (23)4 from | (37)2 decreased, | |
(12)9 different | (24)4 the | (38)2 differed |
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