19) The log-rank test was used to check for significant differences between curves. |
PMID:23294979 DOI:10.1111/vco.12010 |
2015 Veterinary and comparative oncology |
* The value of molecular expression of KIT and KIT ligand analysed using real-time polymerase chain reaction and immunohistochemistry as a prognostic indicator for canine cutaneous mast cell tumours. |
- This study investigated the correlation between KIT gene expression determined by immunohistochemistry and real-time polymerase chain reaction (RT-PCR) and the rate of tumour recurrence and tumour-related deaths in dogs affected with mast cell tumour (MCT). Kaplan-Meier curves were constructed to compare tumour recurrence and tumour-related death between patients. The log-rank test was used to check for significant differences between curves. KIT-I, KIT-II and KIT-III staining patterns were observed in 9 (11.11%), 50 (61.73%) and 22 (27.16%) tumours, respectively. Tumour recurrence rates and tumour-related deaths were not associated with KIT staining patterns (P = 0278, P > 0.05), KIT (P = 0.289, P > 0.05) or KIT ligand (P = 0.106, P > 0.05) gene expression. Despite the lack of association between KIT staining pattern and patient survival time, the results suggest a correlation between aberrant KIT localization and increased proliferative activity of MCTs. RT-PCR seems to be a sensible method for quantitative detection of KIT gene expression in canine MCT, although expressions levels are not correlated with prognosis. |
(1)67 differences | (15)5 correlations | (29)3 influence | (44)2 dose-dependent |
(2)40 increase | (16)5 effects | (30)3 negative | (45)2 elevation |
(3)36 difference | (17)5 for | (31)3 number | (46)2 evidence |
(4)28 decrease | (18)5 increases | (32)3 roles | (47)2 factors |
(5)12 reduction | (19)5 risk | (33)2 (P | (48)2 group |
(6)11 predictors | (20)4 effect | (35)2 alterations | (49)2 inhibition |
(7)10 association | (21)4 in | (36)2 and | (50)2 interaction |
(8)9 improvement | (22)4 reductions | (37)2 at | (51)2 morbidity |
(9)8 *null* | (23)4 relationship | (38)2 between-group | (52)2 positive |
(10)7 changes | (24)3 (p | (39)2 blood | (53)2 prediction |
(11)7 predictor | (25)3 alteration | (40)2 challenge | (54)2 proportion |
(12)7 role | (26)3 as | (41)2 contribution | (55)2 source |
(13)6 correlation | (27)3 higher | (42)2 cytotoxic | (56)2 time |
(14)5 associations | (28)3 improvements | (43)2 decreases |
add keyword