* The selective conversion of glutamic acid in amino acid mixtures using glutamate decarboxylase--a means of separating amino acids for synthesizing biobased chemicals.
- Amino acids (AAs) derived from hydrolysis of protein rest streams are interesting feedstocks for the chemical industry due to their functionality. However, separation of AAs is required before they can be used for further applications. Electrodialysis may be applied to separate AAs, but its efficiency is limited when separating AAs with similar isoelectric points. To aid the separation, specific conversion of an AA to a useful product with different charge behavior to the remaining compounds is desired. Here the separation of L-aspartic acid (Asp) and L-glutamic acid (Glu) was studied. L-Glutamate α-decarboxylase (GAD, Type I, EC 4.1.1.15) was applied to specifically convert Glu into γ-aminobutyric acid (GABA). GABA has a different charge behavior from Asp therefore allowing a potential separation by electrodialysis. Competitive inhibition and reduced operational stability caused by Asp could be eliminated by maintaining a sufficiently high concentration of Glu. Immobilization of GAD does not reduce the enzyme's initial activity. However, the operational stability was slightly reduced. An initial study on the reaction operating in a continuous mode was performed using a column reactor packed with immobilized GAD. As the reaction mixture was only passed once through the reactor, the conversion of Glu was lower than expected. To complete the conversion of Glu, the stream containing Asp and unreacted Glu might be recirculated back to the reactor after GABA has been removed. Overall, the reaction by GAD is specific to Glu and can be applied to aid the electrodialysis separation of Asp and Glu.
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Overview of verb reduce
The verb reduce has 20 senses (first 11 from tagged texts)
1. (102) reduce, cut down, cut back, trim, trim down, trim back, cut, bring down -- (cut down on;
make a reduction in; "reduce your daily fat intake"; "The employer wants to cut back health
benefits")
2. (9) reduce -- (make less complex; "reduce a problem to a single question")
3. (5) reduce -- (bring to humbler or weaker state or condition; "He reduced the population to
slavery")
4. (3) reduce -- (simplify the form of a mathematical equation of expression by substituting one
term for another)
5. (3) reduce -- (lower in grade or rank or force somebody into an undignified situation; "She
reduced her niece to a servant")
6. (3) reduce, come down, boil down -- (be the essential element; "The proposal boils down to a
compromise")
7. (2) shrink, reduce -- (reduce in size; reduce physically; "Hot water will shrink the sweater";
"Can you shrink this image?")
8. (1) reduce -- (lessen and make more modest; "reduce one's standard of living")
9. (1) reduce, scale down -- (make smaller; "reduce an image")
10. (1) deoxidize, deoxidise, reduce -- (to remove oxygen from a compound, or cause to react with
hydrogen or form a hydride, or to undergo an increase in the number of electrons)
11. (1) reduce, tighten -- (narrow or limit; "reduce the influx of foreigners")
12. repress, quash, keep down, subdue, subjugate, reduce -- (put down by force or intimidation; "The
government quashes any attempt of an uprising"; "China keeps down her dissidents very efficiently";
"The rich landowners subjugated the peasants working the land")
13. reduce -- (undergo meiosis; "The cells reduce")
14. reduce -- (reposition (a broken bone after surgery) back to its normal site)
15. reduce -- (destress and thus weaken a sound when pronouncing it)
16. abridge, foreshorten, abbreviate, shorten, cut, contract, reduce -- (reduce in scope while
retaining essential elements; "The manuscript must be shortened")
17. boil down, reduce, decoct, concentrate -- (be cooked until very little liquid is left; "The
sauce should reduce to one cup")
18. reduce, boil down, concentrate -- (cook until very little liquid is left; "The cook reduced the
sauce by boiling it for a long time")
19. dilute, thin, thin out, reduce, cut -- (lessen the strength or flavor of a solution or mixture;
"cut bourbon")
20. reduce, melt off, lose weight, slim, slenderize, thin, slim down -- (take off weight)
Overview of adj reduced
The adj reduced has 2 senses (first 2 from tagged texts)
1. (6) decreased, reduced -- (made less in size or amount or degree)
2. (1) reduced, rock-bottom -- (well below normal (especially in price))
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