* Salt ions and related parameters affect PEI-DNA particle size and transfection efficiency in Chinese hamster ovary cells.
- Transfection efficiency is directly associated with the expression level and quantity of recombinant protein after the transient transfection of animal cells. The transfection process can be influenced by many still-unknown factors, so it is valuable to study the precise mechanism and explore these factors in gene delivery. Polyethylenimine (PEI) is considered to have high transfection efficiency and endosome-disrupting capacity. Here we aimed to investigate optimal conditions for transfection efficiency by setting different parameters, including salt ion concentration, DNA/PEI ratio, and incubation time. We examined the PEI-DNA particle size using a Malvern particle size analyzer and assessed the transfection efficiency using flow cytometry in Chinese hamster ovary-S cells. Salt ions, higher amounts of PEI tended to improve the aggregation of PEI-DNA particles and the particle size of PEI-DNA complexes and the transfection efficiency were increased. Besides, the particle size was also found to benefit from longer incubation time. However, the transfection efficiency increased to maximum of 68.92 % at an incubation time of 10 min, but decreased significantly thereafter to 23.71 %, when incubating for 120 min (P < 0.05). Besides, PEI-DNA complexes formed in salt-free condition were unstable. Our results suggest DNA and PEI incubated in 300 mM NaCl at a ratio of 1:4 for 10 min could achieve the optimal transfection efficiency. Our results might provide guidance for the optimization of transfection efficiency and the industrial production of recombinant proteins.
=>供給する, 与える, 規定する, 条件とする, 準備する, 用意する, 扶養する, 援助する
Overview of verb provide
The verb provide has 7 senses (first 4 from tagged texts)
1. (270) supply, provide, render, furnish -- (give something useful or necessary to; "We provided
the room with an electrical heater")
2. (25) provide, supply, ply, cater -- (give what is desired or needed, especially support, food or
sustenance; "The hostess provided lunch for all the guests")
3. (14) provide -- (determine (what is to happen in certain contingencies), especially by including
a proviso condition or stipulation; "The will provides that each child should receive half of the
money"; "The Constitution provides for the right to free speech")
4. (2) put up, provide, offer -- (mount or put up; "put up a good fight"; "offer resistance")
5. leave, allow for, allow, provide -- (make a possibility or provide opportunity for; permit to be
attainable or cause to remain; "This leaves no room for improvement"; "The evidence allows only one
conclusion"; "allow for mistakes"; "leave lots of time for the trip"; "This procedure provides for
lots of leeway")
6. provide, bring home the bacon -- (supply means of subsistence; earn a living; "He provides for
his large family by working three jobs"; "Women nowadays not only take care of the household but
also bring home the bacon")
7. provide -- (take measures in preparation for; "provide for the proper care of the passengers on
the cruise ship")
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