* Analysis of imprinted gene expression and implantation in haploid androgenetic mouse embryos.
- The successful development of mammalian embryos requires both parental genomes. Nuclear transfer techniques have been adapted to generate uniparental embryos, which possess two sets of paternal or maternal genomes. These embryos fail to develop to term because of abnormal imprinted gene expression, which is not regulated by Mendelian inheritance. Uniparental embryos provide us with an important model to investigate imprinted gene function and ontogenesis. To evaluate the pre- and post-developmental ability of haploid androgenetic mouse embryos, and to analyse the expression of imprinted genes Igf2r, Asb4 and Mest in haploid androgenetic/gynogenetic blastocysts, we produced the haploid mouse embryos using the enucleation technique, examined their development at 6.5 dpc and quantified gene expression by quantitative real-time PCR. The results demonstrated that the developmental potential of haploid embryos was severely impaired and revealed that the haploid androgenones could induce the deciduas reaction, but failed to retain a live foetus at 6.5 dpc. Expression of imprinted genes Igf2r and Asb4 was unregulated in haploid androgenetic/gynogenetic blastocysts.
=>供給する, 与える, 規定する, 条件とする, 準備する, 用意する, 扶養する, 援助する
Overview of verb provide
The verb provide has 7 senses (first 4 from tagged texts)
1. (270) supply, provide, render, furnish -- (give something useful or necessary to; "We provided
the room with an electrical heater")
2. (25) provide, supply, ply, cater -- (give what is desired or needed, especially support, food or
sustenance; "The hostess provided lunch for all the guests")
3. (14) provide -- (determine (what is to happen in certain contingencies), especially by including
a proviso condition or stipulation; "The will provides that each child should receive half of the
money"; "The Constitution provides for the right to free speech")
4. (2) put up, provide, offer -- (mount or put up; "put up a good fight"; "offer resistance")
5. leave, allow for, allow, provide -- (make a possibility or provide opportunity for; permit to be
attainable or cause to remain; "This leaves no room for improvement"; "The evidence allows only one
conclusion"; "allow for mistakes"; "leave lots of time for the trip"; "This procedure provides for
lots of leeway")
6. provide, bring home the bacon -- (supply means of subsistence; earn a living; "He provides for
his large family by working three jobs"; "Women nowadays not only take care of the household but
also bring home the bacon")
7. provide -- (take measures in preparation for; "provide for the proper care of the passengers on
the cruise ship")
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