* Structure elucidation, protein profile and the antitumor effect of the biological active substance extracted from sea cucumber Holothuria polii.
- Holothuria polii (Delle Chiaje, 1823) (Holothuriidae) is a sea cucumber inhabiting Mediterranean Sea coast of Egypt. The bioactive compound of its tegument has antifungal, antibacterial and antiparasitic effects. The present study aims to elucidate the structure of the bioactive material of H. polii for pharmacological and chemotaxonomic purposes. Furthermore, the study demonstrates its efficacy as a cytotoxic agents against two tumor cell lines HCT116 (colon adenocarcinoma cell line) and MCF7 (breast adenocarcinoma cell line). The biological active compound of the ethanol extract has been characterized by means of infrared (IR), proton-nuclear magnetic resonance ((1)H NMR), ultraviolet-visible (UV-Vis) and mass spectra. Protein profile was carried out using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Cytotoxic activity was carried out according to sulforhodamine-B assay. IR, (1)H NMR, UV-Vis and mass spectra showed that the extracted bioactive material is a nonsulfated hexaosides called bivittoside. This glycoside is composed of aglycone and a glycosidic chain (carbohydrate chain) enclosed with six sugar units, including xylose, glucose, 3-O-methylglucose and quinovose. There were no traces of dissolved proteins. The preliminary cytotoxic assay of bivittoside exhibited significant cytotoxic activity against two types of cultured tumor cell lines of HCT116 and MCF7. The half-maximal inhibitory concentration was 17.4 µg/ml and 18 µg/ml for MCF7 and HCT116, respectively. Although H. polii belongs to the genus Holothuria, the lacking of sulfate group and the fact that it contains up to six monosaccharides make it different from this genus. The present study suggests separation of H. polii from its genus to a new one. On the other hand, results support the hypothesis that H. polii bioactive compound has an antitumor effect.
=>出席している, そこにある, 現在の, 今の, 当面の, いあわせている, 考慮している, 現在, を人に贈る,
進呈する, を与える, を渡す, を紹介する, を公開する, を見せる, 示す, 贈り物, 【文法】現在時制の,
プレゼント, 贈る, ある, 現在形の
Overview of noun present
The noun present has 3 senses (first 2 from tagged texts)
1. (17) present, nowadays -- (the period of time that is happening now; any continuous stretch of
time including the moment of speech; "that is enough for the present"; "he lives in the present with
no thought of tomorrow")
2. (4) present -- (something presented as a gift; "his tie was a present from his wife")
3. present, present tense -- (a verb tense that expresses actions or states at the time of speaking)
Overview of verb present
The verb present has 13 senses (first 10 from tagged texts)
1. (40) show, demo, exhibit, present, demonstrate -- (give an exhibition of to an interested
audience; "She shows her dogs frequently"; "We will demo the new software in Washington")
2. (28) present, represent, lay out -- (bring forward and present to the mind; "We presented the
arguments to him"; "We cannot represent this knowledge to our formal reason")
3. (14) stage, present, represent -- (perform (a play), especially on a stage; "we are going to
stage `Othello'")
4. (13) present, submit -- (hand over formally)
5. (7) present, pose -- (introduce; "This poses an interesting question")
6. (3) award, present -- (give, especially as an honor or reward; "bestow honors and prizes at
graduation")
7. (3) give, gift, present -- (give as a present; make a gift of; "What will you give her for her
birthday?")
8. (3) deliver, present -- (deliver (a speech, oration, or idea); "The commencement speaker
presented a forceful speech that impressed the students")
9. (2) introduce, present, acquaint -- (cause to come to know personally; "permit me to acquaint you
with my son"; "introduce the new neighbors to the community")
10. (1) portray, present -- (represent abstractly, for example in a painting, drawing, or sculpture;
"The father is portrayed as a good-looking man in this painting")
11. confront, face, present -- (present somebody with something, usually to accuse or criticize; "We
confronted him with the evidence"; "He was faced with all the evidence and could no longer deny his
actions"; "An enormous dilemma faces us")
12. present -- (formally present a debutante, a representative of a country, etc.)
13. salute, present -- (recognize with a gesture prescribed by a military regulation; assume a
prescribed position; "When the officers show up, the soldiers have to salute")
Overview of adj present
The adj present has 2 senses (first 2 from tagged texts)
1. (60) present -- (temporal sense; intermediate between past and future; now existing or happening
or in consideration; "the present leader"; "articles for present use"; "the present topic"; "the
present system"; "present observations")
2. (39) present -- (being or existing in a specified place; "the murderer is present in this room";
"present at the wedding"; "present at the creation")
--- WordNet end ---