ELIZA

return kwic search for performed out of >500 occurrences
272110 occurrences (No.94 in the rank) during 5 years in the PubMed. [no cache] 500 found

136) In situ hybridization (ISH) against human Alu sequences was performed to distinguish donor osteogenic cells from host cells.

--- ABSTRACT ---

PMID:23038663 DOI:10.1002/term.1619

2015 Journal of tissue engineering and regenerative medicine

* Implantation of osteogenic differentiated donor mesenchymal stem cells causes recruitment of host cells.

- The interaction between host and donor cells is believed to play an important role in osteogenesis. However, it is still unclear how donor osteogenic cells behave and interact with host cells in vivo. The purpose of this study was to track the interactions between transplanted osteogenic cells and host cells during osteogenesis. In vitro migration assay was carried out to investigate the ability of osteogenic differentiated human mesenchymal stem cells (O-hMSCs) to recruit MSCs. At the in vivo level, O-hMSCs were implanted subcutaneously or into skull defects in severe combined immunodeficient (SCID) mice. New bone formation was observed by micro-CT and histological procedures. In situ hybridization (ISH) against human Alu sequences was performed to distinguish donor osteogenic cells from host cells. In vitro migration assay revealed an increased migration potential of MSCs by co-culturing with O-hMSCs. In agreement with the results of in vitro studies, ISH against human Alu sequences showed that host mouse MSCs migrated in large numbers into the transplantation site in response to O-hMSCs. Interestingly, host cells recruited by O-hMSCs were the major cell populations in newly formed bone tissues, indicating that O-hMSCs can trigger and initiate osteogenesis when transplanted in orthotopic sites. The observations from this study demonstrated that in vitro induced O-hMSCs were able to attract host MSCs in vivo and were involved in osteogenesis together with host cells, which may be of importance for bone tissue-engineering applications.

--- ABSTRACT END ---

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[frequency of next (right) word to performed]

(1)60 in	(10)15 for	(19)3 based	(28)2 equally
(2)58 null	(11)13 at	(20)3 of	(29)2 normally
(3)45 to	(12)6 as	(21)3 this	(30)2 procedures
(4)35 using	(13)5 the	(22)2 3	(31)2 successfully
(5)33 on	(14)5 under	(23)2 6	(32)2 three
(6)30 with	(15)5 via	(24)2 better	(33)2 through
(7)23 by	(16)4 an	(25)2 both	(34)2 twice
(8)22 a	(17)4 before	(26)2 but
(9)16 and	(18)3 after	(27)2 daily

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--- WordNet output for performed --- Overview of verb perform The verb perform has 4 senses (first 4 from tagged texts) 1. (25) perform, execute, do -- (carry out or perform an action; "John did the painting, the weeding, and he cleaned out the gutters"; "the skater executed a triple pirouette"; "she did a little dance") 2. (19) perform -- (perform a function; "Who will perform the wedding?") 3. (19) perform -- (give a performance (of something); "Horowitz is performing at Carnegie Hall tonight"; "We performed a popular Gilbert and Sullivan opera") 4. (2) do, perform -- (get (something) done; "I did my job") --- WordNet end ---