130) In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. |
PMID:23349081 DOI:10.1002/term.1682 |
2015 Journal of tissue engineering and regenerative medicine |
* An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering. |
- Regenerative medicine is targeted to improve, restore or replace damaged tissues or organs using a combination of cells, materials and growth factors. Both tissue engineering and developmental biology currently deal with the process of tissue self-assembly and extracellular matrix (ECM) deposition. In this investigation, additive manufacturing (AM) with a multihead deposition system (MHDS) was used to fabricate three-dimensional (3D) cell-printed scaffolds using layer-by-layer (LBL) deposition of polycaprolactone (PCL) and chondrocyte cell-encapsulated alginate hydrogel. Appropriate cell dispensing conditions and optimum alginate concentrations for maintaining cell viability were determined. In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. PCL-alginate gels containing transforming growth factor-β (TGFβ) showed higher ECM formation. The 3D cell-printed scaffolds of PCL-alginate gel were implanted in the dorsal subcutaneous spaces of female nude mice. Histochemical [Alcian blue and haematoxylin and eosin (H&E) staining] and immunohistochemical (type II collagen) analyses of the retrieved implants after 4 weeks revealed enhanced cartilage tissue and type II collagen fibril formation in the PCL-alginate gel (+TGFβ) hybrid scaffold. In conclusion, we present an innovative cell-printed scaffold for cartilage regeneration fabricated by an advanced bioprinting technology. |
(1)60 in | (10)15 for | (19)3 based | (28)2 equally |
(2)58 *null* | (11)13 at | (20)3 of | (29)2 normally |
(3)45 to | (12)6 as | (21)3 this | (30)2 procedures |
(4)35 using | (13)5 the | (22)2 3 | (31)2 successfully |
(5)33 on | (14)5 under | (23)2 6 | (32)2 three |
(6)30 with | (15)5 via | (24)2 better | (33)2 through |
(7)23 by | (16)4 an | (25)2 both | (34)2 twice |
(8)22 a | (17)4 before | (26)2 but | |
(9)16 and | (18)3 after | (27)2 daily |
add keyword