ELIZA

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392826 occurrences (No.45 in the rank) during 5 years in the PubMed. [no cache] 500 found

399) Differentiated cells were observed through dithizone staining, and Pdx1 and insulin expressed in differentiated cells were detected with immunofluorescence.

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PMID:24303870 DOI:10.3109/21691401.2013.864662

2015 Artificial cells, nanomedicine, and biotechnology

* Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells.

- In this study, we explored the possibility of using in vitro differentiation to create functional beta-like islet cells from chicken umbilical cord mesenchymal stem cells (UCMSCs). Passaged UCMSCs were induced to differentiate into pancreatic beta-like islet cells. Differentiated cells were observed through dithizone staining, and Pdx1 and insulin expressed in differentiated cells were detected with immunofluorescence. Insulin and C-peptide production from differentiated cells were analyzed using ELISA and western blotting. Differentiated cells were found to not only express Pdx1, insulin, and C-peptide, but also to display a glucose-responsive secretion of these hormones.

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(1)155 in	(9)9 on	(17)4 a	(25)2 data
(2)48 null	(10)8 by	(18)4 during	(26)2 experimentally
(3)36 that	(11)8 to	(19)4 the	(27)2 reduction
(4)19 for	(12)8 when	(20)3 variation	(28)2 significant
(5)18 at	(13)6 among	(21)2 as	(29)2 through
(6)16 between	(14)6 following	(22)2 changes	(30)2 throughout
(7)11 with	(15)5 after	(23)2 clinical	(31)2 up
(8)9 and	(16)5 from	(24)2 compared	(32)2 using

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--- WordNet output for observed --- Overview of verb observe The verb observe has 9 senses (first 8 from tagged texts) 1. (39) detect, observe, find, discover, notice -- (discover or determine the existence, presence, or fact of; "She detected high levels of lead in her drinking water"; "We found traces of lead in the paint") 2. (17) note, observe, mention, remark -- (make mention of; "She observed that his presentation took up too much time"; "They noted that it was a fine day to go sailing") 3. (12) note, take note, observe -- (observe with care or pay close attention to; "Take note of this chemical reaction") 4. (10) observe -- (watch attentively; "Please observe the reaction of these two chemicals") 5. (4) respect, honor, honour, abide by, observe -- (show respect towards; "honor your parents!") 6. (3) observe, celebrate, keep -- (behave as expected during of holidays or rites; "Keep the commandments"; "celebrate Christmas"; "Observe Yom Kippur") 7. (2) watch, observe, follow, watch over, keep an eye on -- (follow with the eyes or the mind; "Keep an eye on the baby, please!"; "The world is watching Sarajevo"; "She followed the men with the binoculars") 8. (1) observe, keep, maintain -- (stick to correctly or closely; "The pianist kept time with the metronome"; "keep count"; "I cannot keep track of all my employees") 9. observe, keep -- (conform one's action or practice to; "keep appointments"; "she never keeps her promises"; "We kept to the original conditions of the contract") Overview of adj observed The adj observed has 1 sense (first 1 from tagged texts) 1. (7) ascertained, discovered, observed -- (discovered or determined by scientific observation; "variation in the ascertained flux depends on a number of factors"; "the discovered behavior norms"; "discovered differences in achievement"; "no explanation for the observed phenomena") --- WordNet end ---