* The role of oxidative stress in citreoviridin-induced DNA damage in human liver-derived HepG2 cells.
- We hypothesize that citreoviridin (CIT) induces DNA damage in human liver-derived HepG2 cells through an oxidative stress mechanism and that N-acetyl-l-cysteine (NAC) protects against CIT-induced DNA damage in HepG2 cells. CIT-induced DNA damage in HepG2 cells was evaluated by alkaline single-cell gel electrophoresis assay. To elucidate the genotoxicity mechanisms, the level of oxidative DNA damage was tested by immunoperoxidase staining for 8-hydroxydeoxyguanosine (8-OHdG); the intracellular generation of reactive oxygen species (ROS) and reduced glutathione (GSH) were examined; mitochondrial membrane potential and lysosomal membranes' permeability were detected; furthermore, protective effects of NAC on CIT-induced ROS formation and CIT-induced DNA damage were evaluated in HepG2 cells. A significant dose-dependent increment in DNA migration was observed at tested concentrations (2.50-10.00 µM) of CIT. The levels of ROS, 8-OHdG formation were increased by CIT, and significant depletion of GSH in HepG2 cells was induced by CIT. Destabilization of lysosome and mitochondria was also observed in cells treated with CIT. In addition, NAC significantly decreased CIT-induced ROS formation and CIT-induced DNA damage in HepG2 cells. The data indicate that CIT induces DNA damage in HepG2 cells, most likely through oxidative stress mechanisms; that NAC protects against DNA damage induced by CIT in HepG2 cells; and that depolarization of mitochondria and lysosomal protease leakage may play a role in CIT-induced DNA damage in HepG2 cells.
Overview of verb observe
The verb observe has 9 senses (first 8 from tagged texts)
1. (39) detect, observe, find, discover, notice -- (discover or determine the existence, presence,
or fact of; "She detected high levels of lead in her drinking water"; "We found traces of lead in
the paint")
2. (17) note, observe, mention, remark -- (make mention of; "She observed that his presentation took
up too much time"; "They noted that it was a fine day to go sailing")
3. (12) note, take note, observe -- (observe with care or pay close attention to; "Take note of this
chemical reaction")
4. (10) observe -- (watch attentively; "Please observe the reaction of these two chemicals")
5. (4) respect, honor, honour, abide by, observe -- (show respect towards; "honor your parents!")
6. (3) observe, celebrate, keep -- (behave as expected during of holidays or rites; "Keep the
commandments"; "celebrate Christmas"; "Observe Yom Kippur")
7. (2) watch, observe, follow, watch over, keep an eye on -- (follow with the eyes or the mind;
"Keep an eye on the baby, please!"; "The world is watching Sarajevo"; "She followed the men with the
binoculars")
8. (1) observe, keep, maintain -- (stick to correctly or closely; "The pianist kept time with the
metronome"; "keep count"; "I cannot keep track of all my employees")
9. observe, keep -- (conform one's action or practice to; "keep appointments"; "she never keeps her
promises"; "We kept to the original conditions of the contract")
Overview of adj observed
The adj observed has 1 sense (first 1 from tagged texts)
1. (7) ascertained, discovered, observed -- (discovered or determined by scientific observation;
"variation in the ascertained flux depends on a number of factors"; "the discovered behavior norms";
"discovered differences in achievement"; "no explanation for the observed phenomena")
--- WordNet end ---