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375291 occurrences (No.49 in the rank) during 5 years in the PubMed. [no cache] 500 found

92) In this study, we report a quick and simple method for comparing decellularization protocols combining the use of paraffin slices and two-dimensional cell cultures.

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PMID:23172824 DOI:10.1002/term.1658

2015 Journal of tissue engineering and regenerative medicine

* Dewaxed ECM: A simple method for analyzing cell behaviour on decellularized extracellular matrices.

- Decellularization techniques have been used on a wide variety of tissues to create cell-seedable scaffolds for tissue engineering. Finding a suitable decellularization protocol for a certain type of tissue can be laborious, especially when organ perfusion devices are needed. In this study, we report a quick and simple method for comparing decellularization protocols combining the use of paraffin slices and two-dimensional cell cultures. We developed three decellularization protocols for adult murine kidney that yielded decellularized extracellular matrices (ECMs) with varying histological properties. The resulting paraffin-embedded ECM slices were deparaffinized and reseeded with murine embryonic stem cells (mESCs). We analyzed cell attachment four days post seeding via determination of cell numbers, and used quantitative Real-Time PCR 13 days post seeding to measure gene expression levels of two genes associated with renal development, Pax2 and Pou3f3. The three decellularization protocols produced kidney-matrices that showed clearly distinguishable results. We demonstrated that formerly paraffin-embedded decellularized ECMs can effectively influence differentiation of stem cells. This method can be used to identify optimal decellularization protocols for recellularization of three-dimensional tissue-scaffolds with embryonic stem cells and other tissue-specific cell types.

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(2)59 for	(13)6 using	(24)2 among	(35)2 on
(3)39 of	(14)5 has	(25)2 but	(36)2 provided
(4)39 to	(15)5 used	(26)2 by	(37)2 should
(5)37 was	(16)4 showed	(27)2 choice	(38)2 study
(6)26 is	(17)4 which	(28)2 combined	(39)2 the
(7)24 and	(18)3 are	(29)2 could	(40)2 uses
(8)15 in	(19)3 as	(30)2 from	(41)2 we
(9)9 that	(20)3 significantly	(31)2 helped
(10)8 can	(21)2 a	(32)2 involves
(11)7 based	(22)2 against	(33)2 known

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--- WordNet output for method --- =>方法, 筋道, 秩序, 規則正しさ, 順序 Overview of noun method The noun method has 2 senses (first 1 from tagged texts) 1. (95) method -- (a way of doing something, especially a systematic way; implies an orderly logical arrangement (usually in steps)) 2. method acting, method -- (an acting technique introduced by Stanislavsky in which the actor recalls emotions or reactions from his or her own life and uses them to identify with the character being portrayed) --- WordNet end ---