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375291 occurrences (No.49 in the rank) during 5 years in the PubMed. [cache]

30) In this study, the complete mitogenome sequence of cyprinid fish, Hainanese barbel steed, Hemibarbus medius Yue (Teleostei: Cyprinidae) has been amplified and sequenced by employing long polymerase chain reaction method.

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PMID:24460168 DOI:10.3109/19401736.2013.845765

2015 Mitochondrial DNA

* The complete mitochondrial genome of Hainanese barbel steed Hemibarbus medius Yue (Teleostei: Cyprinidae).

- In this study, the complete mitogenome sequence of cyprinid fish, Hainanese barbel steed, Hemibarbus medius Yue (Teleostei: Cyprinidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,614 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region (CR). CR of 933 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of Hemibarbus medius is 29.8% for A, 27.2% for C, 25.9% for T and 17.1% for G, with a slight AT bias of 55.7%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.

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(1)81 null	(12)7 with	(23)2 allowed	(34)2 may
(2)59 for	(13)6 using	(24)2 among	(35)2 on
(3)39 of	(14)5 has	(25)2 but	(36)2 provided
(4)39 to	(15)5 used	(26)2 by	(37)2 should
(5)37 was	(16)4 showed	(27)2 choice	(38)2 study
(6)26 is	(17)4 which	(28)2 combined	(39)2 the
(7)24 and	(18)3 are	(29)2 could	(40)2 uses
(8)15 in	(19)3 as	(30)2 from	(41)2 we
(9)9 that	(20)3 significantly	(31)2 helped
(10)8 can	(21)2 a	(32)2 involves
(11)7 based	(22)2 against	(33)2 known

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--- WordNet output for method --- =>方法, 筋道, 秩序, 規則正しさ, 順序 Overview of noun method The noun method has 2 senses (first 1 from tagged texts) 1. (95) method -- (a way of doing something, especially a systematic way; implies an orderly logical arrangement (usually in steps)) 2. method acting, method -- (an acting technique introduced by Stanislavsky in which the actor recalls emotions or reactions from his or her own life and uses them to identify with the character being portrayed) --- WordNet end ---