* Identification of carriers among individuals recruited in the typhoid registry in Malaysia using stool culture, polymerase chain reaction, and dot enzyme immunoassay as detection tools.
- Chronic carriers of Salmonella Typhi act as reservoirs for the organism and become the agents of typhoid outbreaks in a community. In this study, chronic carriers in Kelantan, Malaysia were first identified using the culture and polymerase chain reaction method. Then, a novel serological tool, designated Typhidot-C, was evaluated in retrospect using the detected individuals as control positives. Chronic carriage positive by the culture and polymerase chain reaction method was recorded at 3.6% (4 out of 110) among individuals who previously had acute typhoid fever and a 9.4% (10 out of 106) carriage rate was observed among food handlers screened during outbreaks. The Typhidot-C assay was able to detect all these positive carriers showing its potential as a viable carrier screening tool and can be used for efficient detection of typhoid carriers in an endemic area. These findings were used to establish the first carrier registry for S Typhi carriers in Malaysia.
=>方法, 筋道, 秩序, 規則正しさ, 順序
Overview of noun method
The noun method has 2 senses (first 1 from tagged texts)
1. (95) method -- (a way of doing something, especially a systematic way; implies an orderly logical
arrangement (usually in steps))
2. method acting, method -- (an acting technique introduced by Stanislavsky in which the actor
recalls emotions or reactions from his or her own life and uses them to identify with the character
being portrayed)
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