ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for low out of >500 occurrences
318584 occurrences (No.68 in the rank) during 5 years in the PubMed. [no cache] 500 found
73) In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins.
--- ABSTRACT ---
PMID:23166055 DOI:10.1002/term.1640
2015 Journal of tissue engineering and regenerative medicine
* Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells.
- Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration. In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins. Three scaffolds were selected for the long-term subculture of miPS cells over two months starting from passages 14 to 29: 1) a gelatin coated polystyrene (Gelatin-PS) that is a widely used scaffold for self-renewal of mouse embryonic stem (mES) cells; 2) a neutral hydrogel poly(N,N-dimethylacrylamide) (PDMAAm); and 3) a negatively charged hydrogel poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS). Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation. The passaged miPS cells maintained a high undifferentiated state on all three scaffolds and a high level of pluripotency by expressing differentiation markers in vitro and forming teratomas in SCID mice with derivatives of all three germ layers. Compared to Gelatin-PS, the two hydrogels exhibited much better self-renewal performance in terms of high proliferation rate and level of expression of undifferentiated gene markers as well as efficiency in pluripotent teratoma formation. Furthermore, the PNaAMPS hydrogel demonstrated a slightly higher efficiency and simpler operation of cell expansion than the PDMAAm hydrogel. To conclude, PNaAMPS hydrogel is an excellent feeder-free scaffold because of its simplicity, low cost and high efficiency in expanding a large number of miPS cells in vitro.
--- ABSTRACT END ---
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[frequency of next (right) word to low]
(1)21 and (18)4 or (35)2 Chilean (52)2 energy
(2)19 laser (19)4 socioeconomic (36)2 HLB (53)2 families
(3)11 doses (20)3 Er:YAG (37)2 OAs (54)2 genetic
(4)11 levels (21)3 O2 (38)2 acculturation (55)2 group
(5)10 *null* (22)3 PFR (39)2 activities (56)2 maternal
(6)10 cost (23)3 PbB (40)2 activity (57)2 method
(7)10 lipoprotein (24)3 STT (41)2 after (58)2 molecular
(8)8 back (25)3 concentrations (42)2 areas (59)2 oral
(9)8 birth (26)3 educational (43)2 at (60)2 pH
(10)8 dose (27)3 features (44)2 bioavailability (61)2 peer
(11)7 in (28)3 risk (45)2 body (62)2 population
(12)7 level (29)3 serum (46)2 cancer (63)2 radiation
(13)6 birthweight (30)3 shear (47)2 children (64)2 resilience
(14)5 but (31)3 tumours (48)2 coverage (65)2 running
(15)4 as (32)3 vagal (49)2 degree (66)2 sensitivity
(16)4 carbon (33)3 water (50)2 density (67)2 settings
(17)4 income (34)3 whole-body (51)2 education (68)2 temperature

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--- WordNet output for low --- =>安い, 低いところ, 卑劣に, 粗食で, 低い(もの), 弱い, 粗末な, 乏しい, 衿ぐりが深い, 建物の低い, 下劣な, 低級な Overview of noun low The noun low has 4 senses (no senses from tagged texts) 1. low, depression -- (an air mass of lower pressure; often brings precipitation; "a low moved in over night bringing sleet and snow") 2. Low, David Low, Sir David Low, Sir David Alexander Cecil Low -- (British political cartoonist (born in New Zealand) who created the character Colonel Blimp (1891-1963)) 3. low -- (a low level or position or degree; "the stock market fell to a new low") 4. first gear, first, low gear, low -- (the lowest forward gear ratio in the gear box of a motor vehicle; used to start a car moving) Overview of verb low The verb low has 1 sense (no senses from tagged texts) 1. moo, low -- (make a low noise, characteristic of bovines) Overview of adj low The adj low has 10 senses (first 6 from tagged texts) 1. (55) low -- (less than normal in degree or intensity or amount; "low prices"; "the reservoir is low") 2. (23) low -- (literal meanings; being at or having a relatively small elevation or upward extension; "low ceilings"; "low clouds"; "low hills"; "the sun is low"; "low furniture"; "a low bow") 3. (11) low, low-toned -- (very low in volume; "a low murmur"; "the low-toned murmur of the surf") 4. (6) low -- (unrefined in character; "low comedy") 5. (4) low, low-pitched -- (used of sounds and voices; low in pitch or frequency) 6. (3) abject, low, low-down, miserable, scummy, scurvy -- (of the most contemptible kind; "abject cowardice"; "a low stunt to pull"; "a low-down sneak"; "his miserable treatment of his family"; "You miserable skunk!"; "a scummy rabble"; "a scurvy trick") 7. humble, low, lowly, modest, small -- (low or inferior in station or quality; "a humble cottage"; "a lowly parish priest"; "a modest man of the people"; "small beginnings") 8. depleted, low -- (no longer sufficient; "supplies are low"; "our funds are depleted") 9. broken, crushed, humbled, humiliated, low -- (subdued or brought low in condition or status; "brought low"; "a broken man"; "his broken spirit") 10. gloomy, grim, blue, depressed, dispirited, down, downcast, downhearted, down in the mouth, low, low-spirited -- (filled with melancholy and despondency ; "gloomy at the thought of what he had to face"; "gloomy predictions"; "a gloomy silence"; "took a grim view of the economy"; "the darkening mood"; "lonely and blue in a strange city"; "depressed by the loss of his job"; "a dispirited and resigned expression on her face"; "downcast after his defeat"; "feeling discouraged and downhearted") Overview of adv low The adv low has 1 sense (first 1 from tagged texts) 1. (3) low -- (in a low position; near the ground; "the branches hung low") --- WordNet end ---