299) In vitro studies revealed that osteoblasts grown on PDGF-BB- and BMP-2-immobilized Hep-Ti increased ALP activity, calcium deposition, osteocalcin and osteopontin levels as compared to those grown on PDGF-BB alone- or BMP-2 alone-immobilized Hep-Ti. |
PMID:23288808 DOI:10.1002/term.1668 |
2015 Journal of tissue engineering and regenerative medicine |
* Co-delivery of platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) coated onto heparinized titanium for improving osteoblast function and osteointegration. |
- The aim of this study was to improve osteoblast function by delivering two growth factors, PDGF-BB and BMP-2, incorporated onto heparinized titanium (Hep-Ti) substrate. To achieve co-delivery of PDGF-BB and BMP-2, the surface of anodized Ti was immobilized with heparin, and then the two growth factors were coated onto the Hep-Ti surface. Incorporation of the two growth factors onto Hep-Ti was evaluated by SEM and XPS. Incorporated PDGF-BB and BMP-2 were released from the Hep-Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on PDGF-BB- and BMP-2-immobilized Hep-Ti increased ALP activity, calcium deposition, osteocalcin and osteopontin levels as compared to those grown on PDGF-BB alone- or BMP-2 alone-immobilized Hep-Ti. These results suggested that co-delivery of PDGF-BB and BMP-2 using Hep-Ti substrate will be a promising material for the enhancement of osteoblast function and osteointegration. |
(1)36 the | (14)5 to | (27)2 RDW | (40)2 likelihood |
(2)34 in | (15)4 after | (28)2 awareness | (41)2 maternal |
(3)27 risk | (16)4 as | (29)2 bone | (42)2 morbidity |
(4)21 *null* | (17)3 activity | (30)2 but | (43)2 number |
(5)20 with | (18)3 among | (31)2 compared | (44)2 over |
(6)16 by | (19)3 at | (32)2 concentrations | (45)2 pre-stimulus |
(7)15 significantly | (20)3 cell | (33)2 drug | (46)2 production |
(8)6 and | (21)3 level | (34)2 during | (47)2 rapidly |
(9)6 expression | (22)3 levels | (35)2 fax | (48)2 use |
(10)6 from | (23)3 physical | (36)2 functional | (49)2 vascular |
(11)5 activation | (24)3 sensitivity | (37)2 hepatic | |
(12)5 odds | (25)2 MI | (38)2 if | |
(13)5 serum | (26)2 Math1 | (39)2 interest |
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