* Primer effect in the detection of mtDNA heteroplasmy: insights from horse Cytochrome b gene.
- Heteroplasmy, the presence of bi-allelic mtDNA types within an individual, has been previously detected in the D-loop region and Cytochrome b gene (Cytb) of mitochondrial DNA using PCR-RFLP. However, heteroplasmy was absent in thousands of equine mtDNA sequences deposited in GenBank. To address whether heteroplasmy widely exists in mitochondria of Chinese indigenous horses, we generated the data set of the target sites in Cytb region with Sanger sequencing and PCR-RFLP method as well. In this study, 23 heteroplasmic individuals were detected in 430 Chinese local horses. Both site and length heteroplasmy were identified in horse Cytb, especially in Xinihe and Ujumqin breeds. Our data provide evidence that the forward and reverse primers seem to produce a similar approximation to the proportion of mutation base call. However, locations of primers affected the proper detection of mtDNA heteroplasmy. The data obtained in this study highlight the importance of the primers in the accurate detection of heteroplasmy.
=>確認飛行物体;略:IFO
Overview of verb identify
The verb identify has 6 senses (first 6 from tagged texts)
1. (32) identify, place -- (recognize as being; establish the identity of someone or something; "She
identified the man on the 'wanted' poster")
2. (7) name, identify -- (give the name or identifying characteristics of; refer to by name or some
other identifying characteristic property; "Many senators were named in connection with the
scandal"; "The almanac identifies the auspicious months")
3. (6) identify -- (consider (oneself) as similar to somebody else; "He identified with the
refugees")
4. (4) identify -- (conceive of as united or associated; "Sex activity is closely identified with
the hypothalamus")
5. (2) identify, discover, key, key out, distinguish, describe, name -- (identify as in botany or
biology, for example)
6. (2) identify -- (consider to be equal or the same; "He identified his brother as one of the
fugitives")
Overview of adj identified
The adj identified has 1 sense (first 1 from tagged texts)
1. (1) identified -- (having the identity known or established; "the identified bodies were released
for burial")
--- WordNet end ---