kwic
higher
higher
right
off
ON
234
1
100
500
strict
ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -
return
kwic search for higher out of >500 occurrences
487192 occurrences (No.29 in the rank) during 5 years in the PubMed. [no cache] 500 found
--- ABSTRACT ---
--- ABSTRACT END ---
[frequency of next (right) word to higher]
487192 occurrences (No.29 in the rank) during 5 years in the PubMed. [no cache] 500 found
| 234) Salt ions, higher amounts of PEI tended to improve the aggregation of PEI-DNA particles and the particle size of PEI-DNA complexes and the transfection efficiency were increased. |
| PMID:24166598 DOI:10.1007/s10616-013-9658-z |
| 2015 Cytotechnology |
| * Salt ions and related parameters affect PEI-DNA particle size and transfection efficiency in Chinese hamster ovary cells. |
| - Transfection efficiency is directly associated with the expression level and quantity of recombinant protein after the transient transfection of animal cells. The transfection process can be influenced by many still-unknown factors, so it is valuable to study the precise mechanism and explore these factors in gene delivery. Polyethylenimine (PEI) is considered to have high transfection efficiency and endosome-disrupting capacity. Here we aimed to investigate optimal conditions for transfection efficiency by setting different parameters, including salt ion concentration, DNA/PEI ratio, and incubation time. We examined the PEI-DNA particle size using a Malvern particle size analyzer and assessed the transfection efficiency using flow cytometry in Chinese hamster ovary-S cells. Salt ions, higher amounts of PEI tended to improve the aggregation of PEI-DNA particles and the particle size of PEI-DNA complexes and the transfection efficiency were increased. Besides, the particle size was also found to benefit from longer incubation time. However, the transfection efficiency increased to maximum of 68.92 % at an incubation time of 10 min, but decreased significantly thereafter to 23.71 %, when incubating for 120 min (P < 0.05). Besides, PEI-DNA complexes formed in salt-free condition were unstable. Our results suggest DNA and PEI incubated in 300 mM NaCl at a ratio of 1:4 for 10 min could achieve the optimal transfection efficiency. Our results might provide guidance for the optimization of transfection efficiency and the industrial production of recombinant proteins. |
[
right
kwic]
(1)69  in |
(17)3 body |
(33)2 by |
(49)2 infant |
| (2)63 than |
(18)3 fatalism |
(34)2 concentrations |
(50)2 initial |
| (3)21 levels |
(19)3 likelihood |
(35)2 coverage |
(51)2 mean |
| (4)17 risk |
(20)3 odds |
(36)2 degree |
(52)2 order |
| (5)10 level |
(21)3 on |
(37)2 different |
(53)2 physiological |
| (6)9 among |
(22)3 quality |
(38)2 dose |
(54)2 probability |
| (7)8 for |
(23)2 (odds |
(39)2 doses |
(55)2 quantity |
| (8)6 rates |
(24)2 *null* | (40)2 drug |
(56)2 rate |
| (9)5 cell |
(25)2 AT |
(41)2 during |
(57)2 relative |
| (10)5 potency |
(26)2 MTBS |
(42)2 education |
(58)2 revision |
| (11)5 scores |
(27)2 RDW |
(43)2 education, |
(59)2 sensitivity |
| (12)4 compared |
(28)2 abstinence |
(44)2 expectations |
(60)2 stress |
| (13)4 number |
(29)2 adjusted |
(45)2 expression |
(61)2 to |
| (14)4 prevalence |
(30)2 amount |
(46)2 frequency |
(62)2 total |
| (15)3 (P |
(31)2 birth |
(47)2 incidence |
(63)2 values |
| (16)3 amounts |
(32)2 blood |
(48)2 individual |
add keyword
--- WordNet output for higher ---