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286252 occurrences (No.88 in the rank) during 5 years in the PubMed. [no cache] 500 found
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286252 occurrences (No.88 in the rank) during 5 years in the PubMed. [no cache] 500 found
| 8) Transient expression of the C/EBPβ major isoform LAP led to an increase in the expression of IL-10 in Siglec-9-expressing cells, but not in mock-transfected control RAW264 cells upon stimulation with LPS, as assessed by either a luciferase assay or the production of IL-10. |
| PMID:24715531 DOI:10.1007/s10616-014-9717-0 |
| 2015 Cytotechnology |
| * Effects of Siglec on the expression of IL-10 in the macrophage cell line RAW264. |
| - Interleukin-10 (IL-10) expression was significantly elevated upon stimulation with lipopolysaccharide (LPS) when the sialic acid-recognizing Ig-superfamily lectin Siglec-5 or -9 was overexpressed in RAW264 cells. During the course to clarify the mechanism for this activation, we found that IL-10 promoter proximal region up to -500 bp led to transactivation similar to that up to -1,500 bp. Among the transcription factors that activate the mouse IL-10 promoter so far reported, the level of C/EBPβ was increased in Siglec-9-expressing cells. Transient expression of the C/EBPβ major isoform LAP led to an increase in the expression of IL-10 in Siglec-9-expressing cells, but not in mock-transfected control RAW264 cells upon stimulation with LPS, as assessed by either a luciferase assay or the production of IL-10. Without LPS, the IL-10 promoter was activated by transiently expressed LAP in Siglec-9-expressing cells, however, the magnitude of transactivation was less than that with the LPS stimulation. The knockdown of C/EBPβ down-regulated the production of IL-10. Taken together, these results suggest that one of the reasons for the stimulation of IL-10 expression in Siglec-9-expressing cells may be an increase in intracellular C/EBPβ level. |
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(1)55  the |
(15)3 key |
(29)2 P-selectin, |
(44)2 cell |
| (2)11 a |
(16)3 multiple |
(30)2 P2X3 |
(45)2 constitutively |
| (3)9 genes |
(17)3 ncRAN |
(32)2 Sema |
(46)2 hyaluronan |
| (4)8 several |
(18)3 nicotine-induced |
(33)2 Siglec-9 |
(47)2 imprinted |
| (5)6 miR-218 |
(19)3 nuclear |
(34)2 TAF4 |
(48)2 intercellular |
| (6)5 these |
(20)3 specific |
(35)2 Wnt |
(49)2 membrane |
| (7)4 VEGF |
(21)3 this |
(36)2 acetyl-CoA |
(50)2 miR-128 |
| (8)4 first |
(22)2 AdipoR1 |
(37)2 adhesion |
(51)2 napsin |
| (9)4 matrix |
(23)2 Caspase |
(38)2 adiponectin |
(52)2 phosphorylated |
| (10)3 CYP1A1 |
(24)2 FBP1 |
(39)2 angiogenic |
(53)2 piRNA-823 |
| (11)3 DNMT1 |
(25)2 GABAA |
(40)2 anti-apoptotic |
(54)2 pro-inflammatory |
| (12)3 all |
(26)2 GLP-1R |
(41)2 b-FGF, |
(55)2 some |
| (13)3 cardiac |
(27)2 Ki-67, |
(42)2 c-Myc |
(56)2 three |
| (14)3 integrin |
(28)2 LOX-1 |
(43)2 cartilage |
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--- WordNet output for expression ---