ELIZA

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286252 occurrences (No.88 in the rank) during 5 years in the PubMed. [no cache] 500 found

78) Additionally, the expression of several lymphangiogenic factors in ADSCs was examined by quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay.

--- ABSTRACT ---

PMID:24401810 DOI:10.1097/SAP.0000000000000084

2015 Annals of plastic surgery

* Adipose-derived stem cells promote proliferation, migration, and tube formation of lymphatic endothelial cells in vitro by secreting lymphangiogenic factors.

- Adipose-derived stem cells (ADSCs) are a promising new therapeutic modality for several diseases and have been applied to various clinical fields because of their multidifferentiation potential and capacity for growth-factor secretion. Recently, 2 in vivo studies showed ADSCs to have potential applications in lymphedema therapy. However, it remains unclear whether ADSCs have direct effects on lymphatic endothelial cells (LECs). In this study, human LECs were treated with murine ADSC-derived conditioned media. Changes in LEC proliferation, migration, and tube formation were assessed by WST-8 assay, transwell chamber assay, and Matrigel-based tube formation assay, respectively, with recombinant human vascular endothelial growth factor-C used as a positive control. Additionally, the expression of several lymphangiogenic factors in ADSCs was examined by quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. Factors secreted by ADSCs induced LEC proliferation, migration, and tube formation more potently than recombinant human vascular endothelial growth factor-C. We confirmed by quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay that some of the lymphangiogenic factors of ADSCs were dramatically up-regulated under serum-starved conditions. These data indicate that ADSCs could directly contribute to lymphangiogenesis via secretory factors in vitro and may thus provide a therapeutic modality for patients with lymphedema.

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(1)55 the	(15)3 key	(29)2 P-selectin,	(44)2 cell
(2)11 a	(16)3 multiple	(30)2 P2X3	(45)2 constitutively
(3)9 genes	(17)3 ncRAN	(32)2 Sema	(46)2 hyaluronan
(4)8 several	(18)3 nicotine-induced	(33)2 Siglec-9	(47)2 imprinted
(5)6 miR-218	(19)3 nuclear	(34)2 TAF4	(48)2 intercellular
(6)5 these	(20)3 specific	(35)2 Wnt	(49)2 membrane
(7)4 VEGF	(21)3 this	(36)2 acetyl-CoA	(50)2 miR-128
(8)4 first	(22)2 AdipoR1	(37)2 adhesion	(51)2 napsin
(9)4 matrix	(23)2 Caspase	(38)2 adiponectin	(52)2 phosphorylated
(10)3 CYP1A1	(24)2 FBP1	(39)2 angiogenic	(53)2 piRNA-823
(11)3 DNMT1	(25)2 GABAA	(40)2 anti-apoptotic	(54)2 pro-inflammatory
(12)3 all	(26)2 GLP-1R	(41)2 b-FGF,	(55)2 some
(13)3 cardiac	(27)2 Ki-67,	(42)2 c-Myc	(56)2 three
(14)3 integrin	(28)2 LOX-1	(43)2 cartilage

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--- WordNet output for expression --- =>表現すること, 式, 表現, 表示, 言い回し, 表現法, 表情 Overview of noun expression The noun expression has 9 senses (first 6 from tagged texts) 1. (23) expression, look, aspect, facial expression, face -- (the feelings expressed on a person's face; "a sad expression"; "a look of triumph"; "an angry face") 2. (18) expression, manifestation, reflection, reflexion -- (expression without words; "tears are an expression of grief"; "the pulse is a reflection of the heart's condition") 3. (15) expression, verbal expression, verbalism -- (the communication (in speech or writing) of your beliefs or opinions; "expressions of good will"; "he helped me find verbal expression for my ideas"; "the idea was immediate but the verbalism took hours") 4. (5) saying, expression, locution -- (a word or phrase that particular people use in particular situations; "pardon the expression") 5. (4) formulation, expression -- (the style of expressing yourself; "he suggested a better formulation"; "his manner of expression showed how much he cared") 6. (4) formula, expression -- (a group of symbols that make a mathematical statement) 7. expression -- ((genetics) the process of expressing a gene) 8. construction, grammatical construction, expression -- (a group of words that form a constituent of a sentence and are considered as a single unit; "I concluded from his awkward constructions that he was a foreigner") 9. expression -- (the act of forcing something out by squeezing or pressing; "the expression of milk from her breast") --- WordNet end ---