241) In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. |
PMID:23349081 DOI:10.1002/term.1682 |
2015 Journal of tissue engineering and regenerative medicine |
* An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering. |
- Regenerative medicine is targeted to improve, restore or replace damaged tissues or organs using a combination of cells, materials and growth factors. Both tissue engineering and developmental biology currently deal with the process of tissue self-assembly and extracellular matrix (ECM) deposition. In this investigation, additive manufacturing (AM) with a multihead deposition system (MHDS) was used to fabricate three-dimensional (3D) cell-printed scaffolds using layer-by-layer (LBL) deposition of polycaprolactone (PCL) and chondrocyte cell-encapsulated alginate hydrogel. Appropriate cell dispensing conditions and optimum alginate concentrations for maintaining cell viability were determined. In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. PCL-alginate gels containing transforming growth factor-β (TGFβ) showed higher ECM formation. The 3D cell-printed scaffolds of PCL-alginate gel were implanted in the dorsal subcutaneous spaces of female nude mice. Histochemical [Alcian blue and haematoxylin and eosin (H&E) staining] and immunohistochemical (type II collagen) analyses of the retrieved implants after 4 weeks revealed enhanced cartilage tissue and type II collagen fibril formation in the PCL-alginate gel (+TGFβ) hybrid scaffold. In conclusion, we present an innovative cell-printed scaffold for cartilage regeneration fabricated by an advanced bioprinting technology. |
(1)21 from | (18)3 amounts | (35)2 compared | (52)2 origins |
(2)15 types | (19)3 aspects | (36)2 dietary | (53)2 output |
(3)10 between | (20)3 cell | (37)2 diseases | (54)2 pHs |
(4)9 doses | (21)3 degrees | (38)2 dosing | (55)2 patterns |
(5)7 concentrations | (22)3 effects | (39)2 ethnic | (56)2 perspectives |
(6)6 time | (23)3 for | (40)2 flow | (57)2 protocols |
(7)5 in | (24)3 health | (41)2 frequencies | (58)2 range |
(8)5 levels | (25)3 mechanisms | (42)2 gene | (59)2 regions |
(9)4 *null* | (26)3 parts | (43)2 genes | (60)2 scenarios |
(10)4 areas | (27)3 roles | (44)2 genetic | (61)2 sensory |
(11)4 combinations | (28)3 species | (45)2 human | (62)2 surface |
(12)4 components | (29)3 ways | (46)2 indicators | (63)2 taxa |
(13)4 groups | (30)2 abutments | (47)2 internal-cone | (64)2 than |
(14)4 methods | (31)2 and | (48)2 laser | (65)2 therapeutic |
(15)4 stages | (32)2 approaches | (49)2 masking | (66)2 tissues |
(16)4 tilt | (33)2 bleaching | (50)2 materials | (67)2 versions |
(17)3 adhesive | (34)2 changes | (51)2 molecular | (68)2 views |
add keyword