106) In vitro studies revealed that osteoblasts grown on PDGF-BB- and BMP-2-immobilized Hep-Ti increased ALP activity, calcium deposition, osteocalcin and osteopontin levels as compared to those grown on PDGF-BB alone- or BMP-2 alone-immobilized Hep-Ti. |
PMID:23288808 DOI:10.1002/term.1668 |
2015 Journal of tissue engineering and regenerative medicine |
* Co-delivery of platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) coated onto heparinized titanium for improving osteoblast function and osteointegration. |
- The aim of this study was to improve osteoblast function by delivering two growth factors, PDGF-BB and BMP-2, incorporated onto heparinized titanium (Hep-Ti) substrate. To achieve co-delivery of PDGF-BB and BMP-2, the surface of anodized Ti was immobilized with heparin, and then the two growth factors were coated onto the Hep-Ti surface. Incorporation of the two growth factors onto Hep-Ti was evaluated by SEM and XPS. Incorporated PDGF-BB and BMP-2 were released from the Hep-Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on PDGF-BB- and BMP-2-immobilized Hep-Ti increased ALP activity, calcium deposition, osteocalcin and osteopontin levels as compared to those grown on PDGF-BB alone- or BMP-2 alone-immobilized Hep-Ti. These results suggested that co-delivery of PDGF-BB and BMP-2 using Hep-Ti substrate will be a promising material for the enhancement of osteoblast function and osteointegration. |
(1)105 the | (10)6 conventional | (19)3 group | (28)2 groups |
(2)23 those | (11)5 controls, | (20)3 pure | (29)2 its |
(3)19 control | (12)5 placebo | (21)2 TCDD | (30)2 long |
(4)14 a | (13)5 untreated | (22)2 age-matched | (31)2 non-Hispanic |
(5)12 that | (14)4 Exo(Normoxic) | (23)2 baseline | (32)2 non-transgenic |
(6)10 controls | (15)4 all | (24)2 bur-prepared | (33)2 people |
(7)10 other | (16)4 patients | (25)2 children | (34)2 plain |
(8)9 healthy | (17)3 an | (26)2 controls), | (35)2 previous |
(9)9 their | (18)3 drug | (27)2 experimental | (36)2 vehicle |
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