* Acute and chronic wound fluids influence keratinocyte function differently.
- Wound healing requires a proper functioning of keratinocytes that migrate, proliferate and lead to a competent wound closure. Impaired wound healing might be due to a disturbed keratinocyte function caused by the wound environment. Basically, chronic wound fluid (CWF) differs from acute wound fluid (AWF). The aim of this study was to analyse the effects of AWF and CWF on keratinocyte function. We therefore investigated keratinocyte migration and proliferation under the influence of AWF and CWF using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test and scratch assay. We further measured the gene expression by qRT-PCR regarding growth factors and matrixmetalloproteinases (MMPs) involved in regeneration processes. AWF had a positive impact on keratinocyte proliferation over time, whereas CWF had an anti-proliferative effect. Keratinocyte migration was significantly impaired by CWF in contrast to an undisturbed wound closure under the influence of AWF. MMP-9 expression was strongly upregulated by CWF compared with AWF. Keratinocyte function was significantly impaired by CWF. An excessive induction of MMP-9 by CWF might lead to a permanent degradation of extracellular matrix and thereby prevent wounds from healing.
Overview of verb compare
The verb compare has 4 senses (first 3 from tagged texts)
1. (57) compare -- (examine and note the similarities or differences of; "John compared his haircut
to his friend's"; "We compared notes after we had both seen the movie")
2. (12) compare -- (be comparable; "This car does not compare with our line of Mercedes")
3. (9) compare, liken, equate -- (consider or describe as similar, equal, or analogous; "We can
compare the Han dynasty to the Romans"; "You cannot equate success in financial matters with greed")
4. compare -- (to form the comparative or superlative form on an adjective or adverb)
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PubMed database: last updated on 2022-05-23 (c)sirasawa 2019