ELIZA

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346933 occurrences (No.53 in the rank) during 5 years in the PubMed. [no cache] 500 found

263) Genotype of the isolates was determined by phylogenetic analysis based on partial F gene sequences.

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PMID:23601048 DOI:10.1111/tbed.12086

2015 Transboundary and emerging diseases

* Pathotypic and genotypic characterization of two Bangladeshi isolates of Newcastle disease virus of chicken and pigeon origin.

- Two Bangladeshi isolates of Newcastle disease virus (NDV), one from a chicken and one from a pigeon, were characterized in this study. Pathogenicity of the isolates was evaluated on the basis of intracerebral pathogenicity index (ICPI). Both the isolates were found to be of velogenic pathotype having ICPI of 1.83 and 1.51 for the chicken and pigeon isolate, respectively. Genotype of the isolates was determined by phylogenetic analysis based on partial F gene sequences. A 766-bp genome fragment spanning partial M and F gene was amplified by RT-PCR and sequenced. The first 354 bp of the coding region of F gene and corresponding deduced amino acid sequences (residues 1-118) of these two NDV isolates were aligned with that of other NDV strains retrieved from GenBank. A phylogenetic tree constructed from the alignment showed that the chicken isolate (BD-C162) belonged to the newly described genotype XIII and the pigeon isolate (BD-P01) to genotype VI. Both the chicken and pigeon isolates possessed a virulent-like fusion protein cleavage site (112) RRQKRF(117) .

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(1)142 the	(8)4 data	(15)2 clinical	(22)2 partial
(2)40 a	(9)4 our	(16)2 combined	(23)2 previous
(3)19 these	(10)4 two	(17)2 current	(24)2 radiological
(4)9 their	(11)3 16S	(18)2 functional	(25)2 sequences
(5)7 this	(12)3 an	(19)2 histological	(26)2 shear
(6)5 its	(13)2 all	(20)2 interviews	(27)2 small
(7)4 both	(14)2 changes	(21)2 morphology	(28)2 surveys

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--- WordNet output for based --- =>に基づき Overview of verb base The verb base has 3 senses (first 1 from tagged texts) 1. (75) establish, base, ground, found -- (use as a basis for; found on; "base a claim on some observation") 2. base -- (situate as a center of operations; "we will base this project in the new lab") 3. free-base, base -- (use (purified cocaine) by burning it and inhaling the fumes) Overview of adj based The adj based has 2 senses (first 2 from tagged texts) 1. (3) based -- (having a base; "firmly based ice") 2. (1) based -- (having a base of operations (often used as a combining form); "a locally based business"; "an Atlanta-based company"; "carrier-based planes") --- WordNet end ---