ELIZA

return kwic search for based on out of >500 occurrences
346933 occurrences (No.53 in the rank) during 5 years in the PubMed. [no cache] 500 found

161) Here we developed a negative feedback regulatory circuit based on a malonyl-CoA-based sensor-actuator.

--- ABSTRACT ---

PMID:24377365 DOI:10.1021/sb400158w

2015 ACS synthetic biology

* Negative feedback regulation of fatty acid production based on a malonyl-CoA sensor-actuator.

- Engineering metabolic biosynthetic pathways has enabled the microbial production of many useful chemicals. However, pathway productivities and yields are often limited by metabolic imbalances. Synthetic regulatory circuits have been shown to be able to balance engineered pathways, improving titers and productivities. Here we developed a negative feedback regulatory circuit based on a malonyl-CoA-based sensor-actuator. Malonyl-CoA is biosynthesized from acetyl-CoA by the acetyl-CoA carboxylase, which is the rate-limiting step for fatty acid biosynthesis. Overexpression of acetyl-CoA carboxylase improves fatty acid production, but slows down cell growth. We have devised a malonyl-CoA sensor-actuator that controls gene expression levels based on intracellular malonyl-CoA concentrations. This sensor-actuator is used to construct a negative feedback circuit to regulate the expression of acetyl-CoA carboxylase. The negative feedback circuit is able to up-regulate acetyl-CoA carboxylase expression when the malonyl-CoA concentration is low and down-regulate acetyl-CoA carboxylase expression when excess amounts of malonyl-CoA have accumulated. We show that the regulatory circuit effectively alleviates the toxicity associated with acetyl-CoA carboxylase overexpression. When used to regulate the fatty acid pathway, the feedback circuit increases fatty acid titer and productivity by 34% and 33%, respectively.

--- ABSTRACT END ---

[
right
kwic]

[frequency of next (right) word to based on]

(1)142 the	(8)4 data	(15)2 clinical	(22)2 partial
(2)40 a	(9)4 our	(16)2 combined	(23)2 previous
(3)19 these	(10)4 two	(17)2 current	(24)2 radiological
(4)9 their	(11)3 16S	(18)2 functional	(25)2 sequences
(5)7 this	(12)3 an	(19)2 histological	(26)2 shear
(6)5 its	(13)2 all	(20)2 interviews	(27)2 small
(7)4 both	(14)2 changes	(21)2 morphology	(28)2 surveys

add keyword

--- WordNet output for based --- =>に基づき Overview of verb base The verb base has 3 senses (first 1 from tagged texts) 1. (75) establish, base, ground, found -- (use as a basis for; found on; "base a claim on some observation") 2. base -- (situate as a center of operations; "we will base this project in the new lab") 3. free-base, base -- (use (purified cocaine) by burning it and inhaling the fumes) Overview of adj based The adj based has 2 senses (first 2 from tagged texts) 1. (3) based -- (having a base; "firmly based ice") 2. (1) based -- (having a base of operations (often used as a combining form); "a locally based business"; "an Atlanta-based company"; "carrier-based planes") --- WordNet end ---