ELIZA

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404793 occurrences (No.42 in the rank) during 5 years in the PubMed. [no cache] 500 found

297) It is concluded that silica, and to a larger extent biosilica, retains its morphogenetic/osteogenic potential after addition to Na-alginate-based hydrogels.

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PMID:23585362 DOI:10.1002/term.1745

2015 Journal of tissue engineering and regenerative medicine

* Development of a morphogenetically active scaffold for three-dimensional growth of bone cells: biosilica-alginate hydrogel for SaOS-2 cell cultivation.

- Polymeric silica is formed from ortho-silicate during a sol-gel formation process, while biosilica is the product of an enzymatically driven bio-polycondensation reaction. Both polymers have recently been described as a template that induces an increased expression of the genes encoding bone morphogenetic protein 2 (BMP-2) and osteoprotegerin in osteoblast-related SaOS-2 cells; simultaneously or subsequently the cells respond with enhanced hydroxyapatite formation. In order to assess whether the biocompatible polymeric silica/biosilica can serve as a morphogenetically active matrix suitable for three-dimensional (3D) cell growth, or even for 3D cell bioprinting, SaOS-2 cells were embedded into a Na-alginate-based hydrogel. Four different gelatinous hydrogel matrices were used for suspending SaOS-2 cells: (a) the hydrogel alone; (b) the hydrogel with 400 μM ortho-silicate; (c) the hydrogel supplemented with 400 μM ortho-silicate and recombinant silicatein to allow biosilica synthesis to occur; and (d) the hydrogel with ortho-silicate and BSA. The SaOS-2 cells showed an increased growth if silica/biosilica components were present in the hydrogel. Likewise intensified was the formation of hydroxyapatite nodules in the silica-containing hydrogels. After an incubation period of 2 weeks, cells present in silica-containing hydrogels showed a significantly higher expression of the genes encoding the cytokine BMP-2, the major fibrillar structural protein collagen 1 and likewise of carbonic anhydrase. It is concluded that silica, and to a larger extent biosilica, retains its morphogenetic/osteogenic potential after addition to Na-alginate-based hydrogels. This property might qualify silica hydrogels to be also used as a matrix for 3D cell printing.

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(1)247 on	(10)3 approach	(19)2 chemotherapy	(28)2 physical
(2)10 method	(11)3 hydrogels	(20)2 computer-tailored	(29)2 rehabilitation
(3)10 therapies	(12)3 information	(21)2 financing	(30)2 retrospective
(4)5 cross-sectional	(13)3 study	(22)2 learning	(31)2 scaffolds
(5)5 upon	(14)3 techniques	(23)2 methods	(32)2 strategy
(6)4 survey	(15)3 therapeutic	(24)2 modelling	(33)2 studies
(7)4 tissue	(16)2 NPWT	(25)2 morphometry	(34)2 telephone
(8)3 CRT	(17)2 RUTF	(26)2 nutrient	(35)2 vaccination
(9)3 anti-bullying	(18)2 and	(27)2 palliative

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--- WordNet output for based --- =>に基づき Overview of verb base The verb base has 3 senses (first 1 from tagged texts) 1. (75) establish, base, ground, found -- (use as a basis for; found on; "base a claim on some observation") 2. base -- (situate as a center of operations; "we will base this project in the new lab") 3. free-base, base -- (use (purified cocaine) by burning it and inhaling the fumes) Overview of adj based The adj based has 2 senses (first 2 from tagged texts) 1. (3) based -- (having a base; "firmly based ice") 2. (1) based -- (having a base of operations (often used as a combining form); "a locally based business"; "an Atlanta-based company"; "carrier-based planes") --- WordNet end ---