kwic
based
based
right
off
ON
284
1
100
500
strict
ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -
return
kwic search for based out of >500 occurrences
404793 occurrences (No.42 in the rank) during 5 years in the PubMed. [no cache] 500 found
--- ABSTRACT ---
--- ABSTRACT END ---
[frequency of next (right) word to based]
404793 occurrences (No.42 in the rank) during 5 years in the PubMed. [no cache] 500 found
| 284) Since extensively expanded ECFCs could still settle on scaffold biomaterials and were able to form tubular structures in Matrigel assays, we conclude that these ex vivo-expanded ECFCs are a novel, very potent cell source for scaffold-based tissue engineering. |
| PMID:23436759 DOI:10.1002/term.1673 |
| 2015 Journal of tissue engineering and regenerative medicine |
| * Human endothelial colony-forming cells expanded with an improved protocol are a useful endothelial cell source for scaffold-based tissue engineering. |
| - One of the major challenges in tissue engineering is to supply larger three-dimensional (3D) bioengineered tissue transplants with sufficient amounts of nutrients and oxygen and to allow metabolite removal. Consequently, artificial vascularization strategies of such transplants are desired. One strategy focuses on endothelial cells capable of initiating new vessel formation, which are settled on scaffolds commonly used in tissue engineering. A bottleneck in this strategy is to obtain sufficient amounts of endothelial cells, as they can be harvested only in small quantities directly from human tissues. Thus, protocols are required to expand appropriate cells in sufficient amounts without interfering with their capability to settle on scaffold materials and to initiate vessel formation. Here, we analysed whether umbilical cord blood (CB)-derived endothelial colony-forming cells (ECFCs) fulfil these requirements. In a first set of experiments, we showed that marginally expanded ECFCs settle and survive on different scaffold biomaterials. Next, we improved ECFC culture conditions and developed a protocol for ECFC expansion compatible with 'Good Manufacturing Practice' (GMP) standards. We replaced animal sera with human platelet lysates and used a novel type of tissue-culture ware. ECFCs cultured under the new conditions revealed significantly lower apoptosis and increased proliferation rates. Simultaneously, their viability was increased. Since extensively expanded ECFCs could still settle on scaffold biomaterials and were able to form tubular structures in Matrigel assays, we conclude that these ex vivo-expanded ECFCs are a novel, very potent cell source for scaffold-based tissue engineering. |
[
right
kwic]
(1)247  on |
(10)3 approach |
(19)2 chemotherapy |
(28)2 physical |
| (2)10 method |
(11)3 hydrogels |
(20)2 computer-tailored |
(29)2 rehabilitation |
| (3)10 therapies |
(12)3 information |
(21)2 financing |
(30)2 retrospective |
| (4)5 cross-sectional |
(13)3 study |
(22)2 learning |
(31)2 scaffolds |
| (5)5 upon |
(14)3 techniques |
(23)2 methods |
(32)2 strategy |
| (6)4 survey |
(15)3 therapeutic |
(24)2 modelling |
(33)2 studies |
| (7)4 tissue |
(16)2 NPWT |
(25)2 morphometry |
(34)2 telephone |
| (8)3 CRT |
(17)2 RUTF |
(26)2 nutrient |
(35)2 vaccination |
| (9)3 anti-bullying |
(18)2 and |
(27)2 palliative |
add keyword
--- WordNet output for based ---