282) Tumour recurrence rates and tumour-related deaths were not associated with KIT staining patterns (P = 0278, P > 0.05), KIT (P = 0.289, P > 0.05) or KIT ligand (P = 0.106, P > 0.05) gene expression. |
PMID:23294979 DOI:10.1111/vco.12010 |
2015 Veterinary and comparative oncology |
* The value of molecular expression of KIT and KIT ligand analysed using real-time polymerase chain reaction and immunohistochemistry as a prognostic indicator for canine cutaneous mast cell tumours. |
- This study investigated the correlation between KIT gene expression determined by immunohistochemistry and real-time polymerase chain reaction (RT-PCR) and the rate of tumour recurrence and tumour-related deaths in dogs affected with mast cell tumour (MCT). Kaplan-Meier curves were constructed to compare tumour recurrence and tumour-related death between patients. The log-rank test was used to check for significant differences between curves. KIT-I, KIT-II and KIT-III staining patterns were observed in 9 (11.11%), 50 (61.73%) and 22 (27.16%) tumours, respectively. Tumour recurrence rates and tumour-related deaths were not associated with KIT staining patterns (P = 0278, P > 0.05), KIT (P = 0.289, P > 0.05) or KIT ligand (P = 0.106, P > 0.05) gene expression. Despite the lack of association between KIT staining pattern and patient survival time, the results suggest a correlation between aberrant KIT localization and increased proliferative activity of MCTs. RT-PCR seems to be a sensible method for quantitative detection of KIT gene expression in canine MCT, although expressions levels are not correlated with prognosis. |
(1)51 the | (16)4 significantly | (31)2 being | (46)2 less |
(2)31 a | (17)3 FIQ | (32)2 diabetes | (47)2 many |
(3)12 increased | (18)3 cancer | (33)2 different | (48)2 maternal |
(4)12 lower | (19)3 chronic | (34)2 early | (49)2 nephrolithiasis |
(5)9 higher | (20)3 more | (35)2 excessive | (50)2 normal |
(6)8 an | (21)3 other | (36)2 faster | (51)2 parenchymal |
(7)7 decreased | (22)3 perceived | (37)2 female | (52)2 physical |
(8)6 poor | (23)3 shorter | (38)2 gene | (53)2 positive |
(9)5 both | (24)3 smoking | (39)2 genetic | (54)2 reductions |
(10)5 greater | (25)3 specific | (40)2 good | (55)2 stress |
(11)4 adverse | (26)2 JS | (41)2 having | (56)2 them |
(12)4 negative | (27)2 SWD | (42)2 high | (57)2 this |
(13)4 poorer | (28)2 alterations | (43)2 implementation | (58)2 undernutrition |
(14)4 reduced | (29)2 altered | (44)2 improvements | (59)2 very |
(15)4 significant | (30)2 any | (45)2 internalizing |
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