ELIZA

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398) Immunofluorescence analysis further indicated that capillary density was evidently increased in the ASCs-ADM group compared with the control or ADM group.

--- ABSTRACT ---

PMID:23038677 DOI:10.1002/term.1622

2015 Journal of tissue engineering and regenerative medicine

* Targeted delivery of adipose-derived stem cells via acellular dermal matrix enhances wound repair in diabetic rats.

- Cell-based therapeutic intervention has emerged as a new approach to accelerate wound closure. Adipose-derived stem cells (ASCs), as a fascinating cell source, have received much attention in tissue repair and regeneration. In this study we evaluated the potential of acellular dermal matrix (ADM) scaffold serving as a carrier for the delivery of ASCs and investigated its therapeutic effects on wound healing. First, ASCs were isolated and characterized for multidifferentiation potential. ASCs-ADM grafts were then prepared, and ADM scaffold was shown to support the in vitro growth and proliferation of ASCs. Next, we analysed paracrine factors in conditioned medium and found that ASCs-ADM grafts secreted various cytokines, including VEGF, HGF, TGFβ and bFGF. Moreover, ASCs-ADM conditioned medium notably stimulated the migration and proliferation of fibroblasts. In vivo, we established an excisional wound model in diabetic rats which received phosphate-buffered saline (PBS), ADM or ASCs-ADM grafts, respectively. Our results demonstrated that implantation of ASCs-ADM significantly enhanced tissue regeneration and increased epithelialization, resulting in accelerated wound closure. Immunofluorescence analysis further indicated that capillary density was evidently increased in the ASCs-ADM group compared with the control or ADM group. In addition, western blot analysis showed that ASCs-ADM significantly increased the expression of angiogenic factors, which was consistent with in vitro data. Taken together, our results suggest that targeted delivery of ASCs via ADM scaffold accelerate diabetic wound healing through a paracrine mechanism, with enhanced granulation tissue formation and increased re-epithelialization and neovascularization.

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(1)124 of	(12)7 is	(23)3 suggested	(34)2 method
(2)63 null	(13)6 with	(24)3 we	(35)2 no
(3)35 was	(14)5 based	(25)3 yielded	(36)2 patients
(4)22 and	(15)5 on	(26)2 (FEA)	(37)2 results
(5)21 showed	(16)5 were	(27)2 allele	(38)2 reveals
(6)19 revealed	(17)4 as	(28)2 because	(39)2 suggests
(7)10 in	(18)4 confirmed	(29)2 by	(40)2 that
(8)9 indicated	(19)4 demonstrated	(30)2 during	(41)2 tools
(9)8 the	(20)4 using	(31)2 found
(10)8 to	(21)3 identified	(32)2 further
(11)7 for	(22)3 shows	(33)2 has

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--- WordNet output for analysis --- =>詳細な検討, 分解, 分析, 解析, 見方, 精神分析 Overview of noun analysis The noun analysis has 6 senses (first 3 from tagged texts) 1. (45) analysis -- (an investigation of the component parts of a whole and their relations in making up the whole) 2. (5) analysis, analytic thinking -- (the abstract separation of a whole into its constituent parts in order to study the parts and their relations) 3. (2) analysis -- (a form of literary criticism in which the structure of a piece of writing is analyzed) 4. analysis -- (the use of closed-class words instead of inflections: e.g., `the father of the bride' instead of `the bride's father') 5. analysis -- (a branch of mathematics involving calculus and the theory of limits; sequences and series and integration and differentiation) 6. psychoanalysis, analysis, depth psychology -- (a set of techniques for exploring underlying motives and a method of treating various mental disorders; based on the theories of Sigmund Freud; "his physician recommended psychoanalysis") --- WordNet end ---