ELIZA

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192) Mating type analysis was carried out for progeny of 13 strains of Lentinula edodes.

--- ABSTRACT ---

PMID:23996277 DOI:10.1002/jobm.201300313

2015 Journal of basic microbiology

* Identification of intralocus recombinants for the mating loci of Lentinula edodes.

- Mating type analysis was carried out for progeny of 13 strains of Lentinula edodes. Out of the 13 strains, one strain HL01 was found with an exceptional phenotype, in that the proportion of incompatibility to compatibility of 132 random pairings of monokaryons derived from the dikaryon was 82:50. This value differs significantly (Chi-square = 11) from the expected 3:1 ratio. The mating types of 189 monokaryons derived from the same sporocarp of HL01 were identified using four standard tester strains. Of the 189 spore monokaryons, 161 monokaryons could be classified into one of four normal mating types (A(1)B(1), A(2)B(2), A(1)B(2), and A(2)B(1)), and the other 28 monokaryons could be classified into another four groups. By crossing in all pairwise combinations, the mating types of the 28 monokaryons were further analyzed. The results indicated that intralocus recombination occurred in both A and B mating loci (matA and matB), at frequencies of 8.5 and 11.6%, respectively. The matA is composed of at least two subloci while the matB may be composed of more than two subloci. The subsequent fruiting test revealed that all compatible pairings which contained at least one of the recombinants had the ability to produce fruiting bodies.

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--- WordNet output for analysis --- =>詳細な検討, 分解, 分析, 解析, 見方, 精神分析 Overview of noun analysis The noun analysis has 6 senses (first 3 from tagged texts) 1. (45) analysis -- (an investigation of the component parts of a whole and their relations in making up the whole) 2. (5) analysis, analytic thinking -- (the abstract separation of a whole into its constituent parts in order to study the parts and their relations) 3. (2) analysis -- (a form of literary criticism in which the structure of a piece of writing is analyzed) 4. analysis -- (the use of closed-class words instead of inflections: e.g., `the father of the bride' instead of `the bride's father') 5. analysis -- (a branch of mathematics involving calculus and the theory of limits; sequences and series and integration and differentiation) 6. psychoanalysis, analysis, depth psychology -- (a set of techniques for exploring underlying motives and a method of treating various mental disorders; based on the theories of Sigmund Freud; "his physician recommended psychoanalysis") --- WordNet end ---