ELIZA

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560012 occurrences (No.18 in the rank) during 5 years in the PubMed. [cache]

146) Gene and protein overexpression was verified by ELISA, RT-PCR and western blot analysis.

--- ABSTRACT ---

PMID:23365046 DOI:10.1002/term.1692

2015 Journal of tissue engineering and regenerative medicine

* Angiogenic gene-modified myoblasts promote vascularization during repair of skeletal muscle defects.

- Vascularization is thought to be a principle obstacle in the reconstruction of skeletal muscle defects. Long-term survival of reconstructed skeletal muscle is dependent on good vascularization. In this study, we upregulated angiogenic gene expression in myoblasts in an attempt to promote vascularization during repair of skeletal muscle defects. Skeletal myoblasts were isolated and expanded from newborn male Sprague-Dawley (SD) rats. The cells were transfected with human vascular endothelial growth factor 165 (VEGF-165) or human stromal cell-derived factor 1 (SDF-1), using Lipofectamine™ 2000 transfection reagent, prior to seeding onto calf collagen scaffolds. Gene and protein overexpression was verified by ELISA, RT-PCR and western blot analysis. Cell-seeded scaffolds were transplanted into back muscle defects in female SD rats. At weeks 2, 4 and 8 after transplantation, Y chromosome detection was used to observe the survival of growth factor-producing cells within the scaffolds in vivo. Capillary density was investigated using microvessel density detection, haematoxylin and eosin (H&E) staining and immunohistochemical staining. We found that vascularization was enhanced by transfected myoblasts compared with non-transfected myoblasts. In addition, VEGF-165 and SDF-1 had a synergistic effect on vascularization during repair of skeletal muscle defects in vivo. In conclusion, we have combined myoblast-seeded collagen sponge with gene therapy, resulting in a promising approach for the construction of well-vascularized skeletal muscle.

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(1)124 of	(12)7 is	(23)3 suggested	(34)2 method
(2)63 null	(13)6 with	(24)3 we	(35)2 no
(3)35 was	(14)5 based	(25)3 yielded	(36)2 patients
(4)22 and	(15)5 on	(26)2 (FEA)	(37)2 results
(5)21 showed	(16)5 were	(27)2 allele	(38)2 reveals
(6)19 revealed	(17)4 as	(28)2 because	(39)2 suggests
(7)10 in	(18)4 confirmed	(29)2 by	(40)2 that
(8)9 indicated	(19)4 demonstrated	(30)2 during	(41)2 tools
(9)8 the	(20)4 using	(31)2 found
(10)8 to	(21)3 identified	(32)2 further
(11)7 for	(22)3 shows	(33)2 has

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--- WordNet output for analysis --- =>詳細な検討, 分解, 分析, 解析, 見方, 精神分析 Overview of noun analysis The noun analysis has 6 senses (first 3 from tagged texts) 1. (45) analysis -- (an investigation of the component parts of a whole and their relations in making up the whole) 2. (5) analysis, analytic thinking -- (the abstract separation of a whole into its constituent parts in order to study the parts and their relations) 3. (2) analysis -- (a form of literary criticism in which the structure of a piece of writing is analyzed) 4. analysis -- (the use of closed-class words instead of inflections: e.g., `the father of the bride' instead of `the bride's father') 5. analysis -- (a branch of mathematics involving calculus and the theory of limits; sequences and series and integration and differentiation) 6. psychoanalysis, analysis, depth psychology -- (a set of techniques for exploring underlying motives and a method of treating various mental disorders; based on the theories of Sigmund Freud; "his physician recommended psychoanalysis") --- WordNet end ---