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475194 occurrences (No.33 in the rank) during 5 years in the PubMed. [no cache] 500 found

246) The preliminary cytotoxic assay of bivittoside exhibited significant cytotoxic activity against two types of cultured tumor cell lines of HCT116 and MCF7.

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PMID:23188650 DOI:10.1177/0748233712466135

2015 Toxicology and industrial health

* Structure elucidation, protein profile and the antitumor effect of the biological active substance extracted from sea cucumber Holothuria polii.

- Holothuria polii (Delle Chiaje, 1823) (Holothuriidae) is a sea cucumber inhabiting Mediterranean Sea coast of Egypt. The bioactive compound of its tegument has antifungal, antibacterial and antiparasitic effects. The present study aims to elucidate the structure of the bioactive material of H. polii for pharmacological and chemotaxonomic purposes. Furthermore, the study demonstrates its efficacy as a cytotoxic agents against two tumor cell lines HCT116 (colon adenocarcinoma cell line) and MCF7 (breast adenocarcinoma cell line). The biological active compound of the ethanol extract has been characterized by means of infrared (IR), proton-nuclear magnetic resonance ((1)H NMR), ultraviolet-visible (UV-Vis) and mass spectra. Protein profile was carried out using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Cytotoxic activity was carried out according to sulforhodamine-B assay. IR, (1)H NMR, UV-Vis and mass spectra showed that the extracted bioactive material is a nonsulfated hexaosides called bivittoside. This glycoside is composed of aglycone and a glycosidic chain (carbohydrate chain) enclosed with six sugar units, including xylose, glucose, 3-O-methylglucose and quinovose. There were no traces of dissolved proteins. The preliminary cytotoxic assay of bivittoside exhibited significant cytotoxic activity against two types of cultured tumor cell lines of HCT116 and MCF7. The half-maximal inhibitory concentration was 17.4 µg/ml and 18 µg/ml for MCF7 and HCT116, respectively. Although H. polii belongs to the genus Holothuria, the lacking of sulfate group and the fact that it contains up to six monosaccharides make it different from this genus. The present study suggests separation of H. polii from its genus to a new one. On the other hand, results support the hypothesis that H. polii bioactive compound has an antitumor effect.

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(1)70 of	(13)6 patterns	(25)3 but	(37)2 functional
(2)54 null	(14)5 may	(26)3 changes	(38)2 increased
(3)49 and	(15)5 using	(27)3 for	(39)2 induces
(4)45 in	(16)5 with	(28)3 measured	(40)2 level
(5)17 was	(17)4 at	(29)3 through	(41)2 or
(6)11 against	(18)4 levels	(30)2 Questionnaire	(42)2 over
(7)10 during	(19)4 on	(31)2 after	(43)2 promotion
(8)9 (PA)	(20)4 which	(32)2 between	(44)2 resumption
(9)8 is	(21)4 while	(33)2 calcium	(45)2 than
(10)7 to	(22)3 among	(34)2 compared	(46)2 that
(11)7 were	(23)3 are	(35)2 decreased	(47)2 via
(12)6 by	(24)3 as	(36)2 diary	(48)2 when

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--- WordNet output for activity --- =>活動, 活躍, 働き, 動き, 活気 Overview of noun activity The noun activity has 6 senses (first 3 from tagged texts) 1. (43) activity -- (any specific behavior; "they avoided all recreational activity") 2. (36) action, activity, activeness -- (the state of being active; "his sphere of activity"; "he is out of action") 3. (13) bodily process, body process, bodily function, activity -- (an organic process that takes place in the body; "respiratory activity") 4. activity -- ((chemistry) the capacity of a substance to take part in a chemical reaction; "catalytic activity") 5. natural process, natural action, action, activity -- (a process existing in or produced by nature (rather than by the intent of human beings); "the action of natural forces"; "volcanic activity") 6. activeness, activity -- (the trait of being active; moving or acting rapidly and energetically; "the level of activity declines with age") --- WordNet end ---